LOCUS       Exported                3019 bp ds-DNA     circular SYN 06-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.seq.xdna [2626 nt] : (EcoRV[413] / 
            EcoRV[413]) to Sequence Window #4 [393 nt] : (Uncut 5'[0] / Uncut 
            3'[393])
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..808
                     /label=BR29_00043011
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [393 nt] : (Uncut 5'[0] / Uncut 3'[393])"
     primer_bind     complement(843..859)
                     /label=M13Rv
     CDS             2004..2819
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgtgtgatg atgctcatgc tgttgaagat actaataata atagaagatc
      481 ttgttattat actatttttc atagagctgg tagagaatgg agacaaatta gacaaactat
      541 tactcaaatt actccaaatt gtgttgttaa taatattaga ggtattccaa aattttgggt
      601 tgatgatgat tgtactccaa attatcaaaa atctgaagaa gtttatatta gagttactgc
      661 tgaaggtgct aaaaattatc cagaaccacc aggttttcca tctggttgtt ctttgagagg
      721 tagaccaggt tctaaatgga aatcttgggg tgaattgact ccattgaaac aaccaatgag
      781 aagaggttga gcttagagac caagttccat cccaatggcg cgccgagctt ggctcgagca
      841 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      901 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      961 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1021 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1081 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1141 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1201 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1261 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1321 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1381 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1441 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1501 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1561 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1621 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1681 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1741 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1801 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1861 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1921 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1981 tatgagtaaa cttggtctga cagttagaaa aactcatcga gcatcaaatg aaactgcaat
     2041 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     2101 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     2161 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     2221 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct
     2281 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     2341 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     2401 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     2461 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccagggatc
     2521 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     2581 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     2641 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     2701 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     2761 tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata
     2821 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     2881 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     2941 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3001 atcacgaggc cctttcgtc
//
