LOCUS       Exported                2989 bp ds-DNA     circular SYN 06-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.seq.xdna [2626 nt] : (EcoRV[413] / 
            EcoRV[413]) to Sequence Window #4 [363 nt] : (Uncut 5'[0] / Uncut 
            3'[363])
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..778
                     /label=BR29_00052161
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [363 nt] : (Uncut 5'[0] / Uncut 3'[363])"
     primer_bind     complement(813..829)
                     /label=M13Rv
     CDS             1974..2789
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atggctgatg ttgaaccacc aggtgcttct gttaaaccaa ctgaagctgc
      481 tagaagaaga gtttttaaaa ttgatggtga agaatttact ccattggaaa ctgattgttt
      541 gattagaatt tttaaaaata aaaaaactgt tcatgctttg tttgttccac caggtactga
      601 attggattgg agattggatc ataatcatta tgctgttggt attaaacata attgtgatat
      661 tattattggt tggaaaccat cttcttggag aattgaaggt gataaagttt cttctttggc
      721 tgatggtttt tctgttgtta ctactaaatc tactgattga gcttagagac caagttccat
      781 cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt gtgaaattgt
      841 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      901 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      961 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1021 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1081 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1141 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1201 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1261 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1321 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1381 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1441 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1501 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1561 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1621 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1681 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1741 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1801 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1861 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1921 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttagaaa
     1981 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     2041 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     2101 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     2161 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     2221 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     2281 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     2341 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     2401 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     2461 aatacctgga atgctgtttt cccagggatc gcagtggtga gtaaccatgc atcatcagga
     2521 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     2581 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     2641 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     2701 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     2761 caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta ttgaagcatt
     2821 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2881 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2941 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
