LOCUS       Exported                2803 bp ds-DNA     circular SYN 06-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.seq.xdna [2626 nt] : (EcoRV[413] / 
            EcoRV[413]) to Sequence Window #4 [177 nt] : (Uncut 5'[0] / Uncut 
            3'[177])
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..592
                     /label=BR29_00096401
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [177 nt] : (Uncut 5'[0] / Uncut 3'[177])"
     primer_bind     complement(627..643)
                     /label=M13Rv
     CDS             1788..2603
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgattccat ctccaggtaa agttgatttg attcaagatg ttagattgca
      481 aactagaact attacttctc catctactaa tcatgatgtt aattctcata atattagaag
      541 aggtttggct tctttggctt tgggtgctac ttgagcttag agaccaagtt ccatcccaat
      601 ggcgcgccga gcttggctcg agcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      661 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      721 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      781 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      841 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1441 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1681 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1741 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca
     1801 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     1861 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     1921 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     1981 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     2041 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     2101 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     2161 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     2221 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     2281 tggaatgctg ttttcccagg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     2341 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     2401 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     2461 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     2521 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac
     2581 gtttcccgtt gaatatggct catactcttc ctttttcaat attattgaag catttatcag
     2641 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2701 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2761 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
