LOCUS       Exported                2934 bp ds-DNA     circular SYN 11-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 11, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan [2626 nt] : (EcoRV[413] / EcoRV[413]) to 
            Untitled Sequence #1 [308 nt] : (Uncut 5'[0] / Uncut 3'[308])
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..723)
                     /label=insert from Untitled Sequence #1
                     /note="note=""Fragment extracted from Untitled Sequence #1 
                     [308 nt] : (Uncut 5'[0] / Uncut 3'[308])"
     primer_bind     complement(758..774)
                     /label=M13Rv
     CDS             complement(1919..2734)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatttggt
      421 ctctaatgtt gccaactact agatctttgt ctgaaaattc tttggatgaa ggtactccaa
      481 gattggttcc aaggagacat ttggatgaat tgaaaagatt ggaatgtgct gcttgtggtt
      541 atagatctta ttctttggaa gttatgttga atcatgaatg tgattgggat tctagaaata
      601 atggtgaatg ggaatattat gtttctgatt ctgatgcttc tgatcatcat tgttcttctg
      661 attcttcttc tgatgaatct gatgatgatg attgtaaaaa ttctggttga gcttagagac
      721 caaatcccaa tggcgcgccg agcttggctc gagcatggtc atagctgttt cctgtgtgaa
      781 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      901 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      961 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1561 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1861 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1921 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     1981 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     2041 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     2101 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     2161 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     2221 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     2281 cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     2341 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     2401 cttctaatac ctggaatgct gttttcccag ggatcgcagt ggtgagtaac catgcatcat
     2461 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     2521 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     2581 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     2641 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     2701 tagagcaaga cgtttcccgt tgaatatggc tcatactctt cctttttcaa tattattgaa
     2761 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2821 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     2881 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
