LOCUS       Exported                2962 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [336 nt] : (Uncut 5'[0] / Uncut 3'[336])
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     misc_feature    416..751
                     /label=BR29_00105921
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [336 nt] : (Uncut 5'[0] / Uncut 3'[336])"
     primer_bind     complement(786..802)
                     /label=M13Rv
     CDS             complement(1947..2762)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atggataata ttacttttgg tactggttct actggtggta gatgttgtga
      481 tcatggtgtt tctgatccat ctgaaacttg taaaaaaaaa ggtttgaact cttatggttg
      541 tactgattgg tcttattctg ctaaagatgg tggtgatttt ttgtattttc caaaaggtgg
      601 ttgtgatgtt gatactgtta aaaattggcc agttggtaga gatgttttgg attttattcc
      661 aggttctgtt gttactcatt tgaaagaaaa aacttctgat ttggaagttg gttttattgg
      721 ttgtgctaat tgagcttaga gaccaagttc catcccaatg gcgcgccgag cttggctcga
      781 gcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1381 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1621 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1741 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1801 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1921 atatatgagt aaacttggtc tgacagttag aaaaactcat cgagcatcaa atgaaactgc
     1981 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     2041 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     2101 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     2161 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     2221 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     2281 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     2341 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     2401 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccaggg
     2461 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     2521 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     2581 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     2641 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     2701 gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc
     2761 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2821 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2881 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2941 cgtatcacga ggccctttcg tc
//
