LOCUS       Exported                2860 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [234 nt] : (Uncut 3'[234] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..649
                     /label=BR29_00106461
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [234 nt] : (Uncut 3'[234] / Uncut 5'[0])"
     primer_bind     complement(684..700)
                     /label=M13Rv
     CDS             complement(1845..2660)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaaact ggatcagcag acaaccattc tggacctggt ttaccattag
      481 ccaaaataac ttgacatcta gaatcttgag catgaacaac caattcagta ccatcagcaa
      541 taatagtatc aatttcacca attctaacaa caccaacttg tctcctagat ttattagaac
      601 cagtaaaata agcacaaaat tctggagcag ccattagaga ccaagttcca tcccaatggc
      661 gcgccgagct tggctcgagc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      721 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      781 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      841 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      901 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      961 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1021 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1081 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1141 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1201 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1261 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1321 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1381 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1441 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1501 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1561 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1621 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1681 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1741 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1801 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa aaactcatcg
     1861 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     1921 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     1981 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     2041 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     2101 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     2161 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     2221 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     2281 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     2341 aatgctgttt tcccagggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     2401 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     2461 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     2521 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     2581 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     2641 tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2761 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     2821 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
