LOCUS       Exported                2902 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [276 nt] : (Uncut 5'[0] / Uncut 3'[276])
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..691
                     /label=BR29_00107301
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [276 nt] : (Uncut 5'[0] / Uncut 3'[276])"
     primer_bind     complement(726..742)
                     /label=M13Rv
     CDS             complement(1887..2702)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgattccat tgggtccaca acaacatggt actgttgctg ctgctaataa
      481 agctggtcat gctaaagctg gtaattgtgt ttgtccacca agaagaggtg gtttgtctaa
      541 aaatagatct gctagattgt ctgctagaac tgatgatgat aatactccac aaggttttca
      601 atgtccaaat tgtcatcata attctcattt ttgtgatgat ggttcttgtg atactcatac
      661 tactacttgg tgagcttaga gaccaagttc catcccaatg gcgcgccgag cttggctcga
      721 gcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1561 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1861 atatatgagt aaacttggtc tgacagttag aaaaactcat cgagcatcaa atgaaactgc
     1921 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     1981 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     2041 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     2101 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     2161 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     2221 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     2281 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     2341 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccaggg
     2401 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     2461 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     2521 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     2581 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     2641 gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc
     2701 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2761 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2821 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2881 cgtatcacga ggccctttcg tc
//
