LOCUS       Exported                2914 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [288 nt] : (Uncut 5'[0] / Uncut 3'[288])
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..703
                     /label=BR29_00112111
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [288 nt] : (Uncut 5'[0] / Uncut 3'[288])"
     primer_bind     complement(738..754)
                     /label=M13Rv
     CDS             complement(1899..2714)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atggcttctg atccatattt ttctccacca tctgaatctc aaaaatgttt
      481 ttatactatt tatagagttg aatctattaa tggtgaaaaa gttttggtta gagaaggtgt
      541 tgatcattct tatactggtt gggatgctga aattaaaaaa attaatgttc attttgataa
      601 agaatgtaat ccacaatatc aatcttctca atgggttttt tttaaaggtg ttagaaaaga
      661 aagacatatt tatccagata gatgagctta gagaccaagt tccatcccaa tggcgcgccg
      721 agcttggctc gagcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      781 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      841 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      901 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1021 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1141 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1201 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1261 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1321 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1381 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1441 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1501 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1561 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1621 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1681 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1741 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1801 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1861 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt agaaaaactc atcgagcatc
     1921 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     1981 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     2041 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     2101 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     2161 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     2221 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     2281 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     2341 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     2401 gttttcccag ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     2461 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     2521 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     2581 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     2641 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
     2701 tgaatatggc tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2761 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2821 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2881 tataaaaata ggcgtatcac gaggcccttt cgtc
//
