LOCUS       Exported                2944 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [318 nt] : (Uncut 3'[318] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..733)
                     /label=BR29_00117591
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [318 nt] : (Uncut 3'[318] / Uncut 5'[0])"
     primer_bind     complement(768..784)
                     /label=M13Rv
     CDS             complement(1929..2744)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaccat ggagcttctc taacatttct accatatctt tcaaatcttt
      481 cagtccaacc attataaaca ttacaaactg gtctaactgg agctggaaaa ccccaaacaa
      541 catgaacatg caaatgtggt ggaatagtac cagaaatttt ttcagtaata catcttctac
      601 caactttcaa aacaaaatca taacctggaa aagaaacagt tcttctaatt ctaaatggtt
      661 caaaacccaa aattggtttt ctaccatcat ttctcaacaa aacacccaaa cattgcatta
      721 gagaccaagt tccatcccaa tggcgcgccg agcttggctc gagcatggtc atagctgttt
      781 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      841 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      901 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      961 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1321 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1381 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1441 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1501 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1561 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1621 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1681 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1741 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1801 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1861 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1921 tctgacagtt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     1981 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     2041 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     2101 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     2161 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     2221 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     2281 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     2341 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     2401 tcaggatatt cttctaatac ctggaatgct gttttcccag ggatcgcagt ggtgagtaac
     2461 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     2521 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     2581 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     2641 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     2701 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt cctttttcaa
     2761 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2821 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     2881 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     2941 cgtc
//
