LOCUS       Exported                2929 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [303 nt] : (Uncut 3'[303] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..718)
                     /label=BR29_00119471
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [303 nt] : (Uncut 3'[303] / Uncut 5'[0])"
     primer_bind     complement(753..769)
                     /label=M13Rv
     CDS             complement(1914..2729)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcattct ctatgagtac ctggaacatt attccaaacc aaagtatcag
      481 tttctttcaa atattgtgga taatcttcag aaatcaaatc agtcaaatat tgtggttcac
      541 caactctttc acctggtggt tgatgacctg gatatggttt ccaagcagca gcttgagtat
      601 gatgatatgg aacttcaact tgtggttgtg gttgtggtgg tggagcagaa ccaccttttt
      661 taccagattt accagtaaac aatctcaaaa ctggattttt cattagagac caagttccat
      721 cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt gtgaaattgt
      781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
      841 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
      901 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
      961 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1021 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1081 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1141 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1201 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1261 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1321 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1381 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1441 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1501 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1561 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     1621 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1681 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     1741 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1801 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1861 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttagaaa
     1921 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     1981 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     2041 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     2101 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     2161 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     2221 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     2281 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     2341 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     2401 aatacctgga atgctgtttt cccagggatc gcagtggtga gtaaccatgc atcatcagga
     2461 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     2521 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     2581 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     2641 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     2701 caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta ttgaagcatt
     2761 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2821 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2881 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
