LOCUS       Exported                2905 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [279 nt] : (Uncut 5'[0] / Uncut 3'[279])
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..694
                     /label=BR29_00119511
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [279 nt] : (Uncut 5'[0] / Uncut 3'[279])"
     primer_bind     complement(729..745)
                     /label=M13Rv
     CDS             complement(1890..2705)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgcataaat gtgttattaa aagagttttt tctgatggtt cttctgttaa
      481 aattccagct caaccagctc aatctactac tatttctaaa caagctgttt gggtttatgg
      541 taattgtcaa ccatttccag ttgttttgaa tgatggttct aaattgtctg gtgaaattga
      601 aactccagct gttactccag ttgaatctgt tgatgaagat gattgttttg aattggatat
      661 ttctggtcaa agatgagctt agagaccaag ttccatccca atggcgcgcc gagcttggct
      721 cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      781 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      841 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1561 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1801 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1861 agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat caaatgaaac
     1921 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1981 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     2041 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     2101 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     2161 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2221 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2281 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     2341 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccca
     2401 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2461 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     2521 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     2581 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     2641 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     2701 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2761 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2821 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2881 aggcgtatca cgaggccctt tcgtc
//
