LOCUS       Exported                3271 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [645 nt] : (Uncut 5'[0] / Uncut 3'[645])
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..1060
                     /label=BR29_00125251
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [645 nt] : (Uncut 5'[0] / Uncut 3'[645])"
     primer_bind     complement(1095..1111)
                     /label=M13Rv
     CDS             complement(2256..3071)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgggttgga ctggtaaatc tgaagcttct aatagaccac caccaggtag
      481 gagaccagaa gaaactactt ggtttacttc tgatccatct tatgcttgtg gtgttaaaat
      541 ttttagacca ggttctgaag gttcttctgg tttggctgaa attactcatt tgaattttca
      601 tgaaaaaatt aaagttttga aaggtcaata tgaatgtgaa gttggtccat cttgttctac
      661 tttgagatgt actatgccaa gaggttggag attggatatt tttggtaaat cttttccaac
      721 tagaagaact acttattttg ctgaaaatgg taatccagaa tataatactt tggcttctga
      781 agctaatatt ccattgtatg attgtagagt tgttattaga tttccaccaa ctggtggtag
      841 agatactggt ccattggctg aagctactca ttttaatttg ggtgaagttg ttggtgtttt
      901 gggtggtaga catacttgtc aagctcaagg tcaatgtgct aatgttaatt gtaatgttcc
      961 aaaaggttat acttattatt ctttgactgg tggtccattt caaactaaag aaaaatctca
     1021 tacttttcaa tctgatgctt gagcttagag accaagttcc atcccaatgg cgcgccgagc
     1081 ttggctcgag catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1141 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     1201 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1261 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1321 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1381 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1441 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1501 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1561 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1621 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1681 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1741 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1801 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1861 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1921 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1981 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2041 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2101 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2161 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2221 atctaaagta tatatgagta aacttggtct gacagttaga aaaactcatc gagcatcaaa
     2281 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     2341 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2401 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     2461 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     2521 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     2581 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     2641 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     2701 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     2761 ttcccaggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     2821 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     2881 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     2941 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     3001 tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga
     3061 atatggctca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3121 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3181 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     3241 aaaaataggc gtatcacgag gccctttcgt c
//
