LOCUS       Exported                2965 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [339 nt] : (Uncut 5'[0] / Uncut 3'[339])
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..754
                     /label=BR32_00031651
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [339 nt] : (Uncut 5'[0] / Uncut 3'[339])"
     primer_bind     complement(789..805)
                     /label=M13Rv
     CDS             complement(1950..2765)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgaactctt tgccaattca accaagagaa gatacttcta attttaaagg
      481 tattactggt gaaatttgtt gtaatagagg tactactgat ccaaataatt tgtgtcatga
      541 tgctaaattg aatgcttatt gttgttctgc tattccaaat gataaaaaag atgaaccagg
      601 tgatgctggt ggttgtgatc aaattgctgc ttttccagtt ggtagaaatg ttactttggt
      661 tgatccaaaa ccaggtttga attgtaaatc tggtagattt aatgatgttc caggttttgt
      721 tggttgtgct caatgagctt agagaccaag ttccatccca atggcgcgcc gagcttggct
      781 cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      841 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      901 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      961 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1021 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1081 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1141 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1201 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1681 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1741 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1801 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1861 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1921 agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat caaatgaaac
     1981 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     2041 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     2101 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     2161 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     2221 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2281 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2341 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     2401 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccca
     2461 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2521 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     2581 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     2641 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     2701 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     2761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2881 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2941 aggcgtatca cgaggccctt tcgtc
//
