LOCUS       Exported                2947 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [321 nt] : (Uncut 5'[0] / Uncut 3'[321])
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..736
                     /label=BR32_00066181
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [321 nt] : (Uncut 5'[0] / Uncut 3'[321])"
     primer_bind     complement(771..787)
                     /label=M13Rv
     CDS             complement(1932..2747)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgggttggc cattgactgc tgattgtaat atttatccaa aaattggtga
      481 taaatctgtt ggttggggtt ctggttttat gggttctgtt gctcatgtta gaattgaaaa
      541 agttgattat gctgttgctt tgtggaaaag agtttgtaaa gctgttaaat tggaagaatt
      601 gccaccaggt gttgaattgt ttgctgaacc aaatactttg agaagaaatc cacaaccagt
      661 tcaaagaaga ggtttggttg gtagagaacc attgggtggt ttggaattgc aagaatgagc
      721 ttagagacca agttccatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg
      781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      901 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1801 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1861 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1921 tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1981 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     2041 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     2101 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     2161 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     2221 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     2281 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     2341 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2401 gaatcaggat attcttctaa tacctggaat gctgttttcc cagggatcgc agtggtgagt
     2461 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2521 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2581 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2641 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2701 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt
     2761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2881 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     2941 tttcgtc
//
