LOCUS       Exported                2785 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [159 nt] : (Uncut 3'[159] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..574)
                     /label=BR32_00101991
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [159 nt] : (Uncut 3'[159] / Uncut 5'[0])"
     primer_bind     complement(609..625)
                     /label=M13Rv
     CDS             complement(1770..2585)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaaaca caacctttca attcatgatg atcttgaaca cctggttgat
      481 catgcaaagc tctagtagac aacaatctag caacatgtgg acaagtttct ttagaatttc
      541 tatgttgagc attatccatt agagaccaag ttccatccca atggcgcgcc gagcttggct
      601 cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      661 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      721 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      781 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      901 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      961 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1201 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1441 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1741 agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat caaatgaaac
     1801 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1861 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1921 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1981 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     2041 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2101 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2161 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     2221 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccca
     2281 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2341 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     2401 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     2461 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     2521 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     2581 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2641 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2701 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2761 aggcgtatca cgaggccctt tcgtc
//
