LOCUS       Exported                2884 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [258 nt] : (Uncut 5'[0] / Uncut 3'[258])
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..673
                     /label=BR32_00117431
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [258 nt] : (Uncut 5'[0] / Uncut 3'[258])"
     primer_bind     complement(708..724)
                     /label=M13Rv
     CDS             complement(1869..2684)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgactccag ttgtttgtaa atatgttgtt tatgatgcta atactccaaa
      481 aaatgttatt ttgagaggtg aagctccagc tggtgaatct cataatattt tgggtgtttg
      541 ggttactttt tcttctaaag attgtgaagc tacttatgat actaaaaatg gtagaatttc
      601 tattattggt gctagaattg gtggtaaacc agttccagtt gatttttctg cttgagctta
      661 gagaccaagt tccatcccaa tggcgcgccg agcttggctc gagcatggtc atagctgttt
      721 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      781 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      841 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
      901 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      961 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1021 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1081 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1141 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1201 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1261 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1321 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1381 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1441 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1501 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1561 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1621 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1681 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1741 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1801 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1861 tctgacagtt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     1921 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     1981 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     2041 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     2101 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     2161 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     2221 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     2281 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     2341 tcaggatatt cttctaatac ctggaatgct gttttcccag ggatcgcagt ggtgagtaac
     2401 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     2461 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     2521 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     2581 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     2641 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt cctttttcaa
     2701 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     2761 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     2821 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     2881 cgtc
//
