LOCUS       Exported                3016 bp ds-DNA     circular SYN 13-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 13, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [390 nt] : (Uncut 5'[0] / Uncut 3'[390])
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..805
                     /label=BR32_00120141
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [390 nt] : (Uncut 5'[0] / Uncut 3'[390])"
     primer_bind     complement(840..856)
                     /label=M13Rv
     CDS             complement(2001..2816)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgggtagaa aatggtttaa taaaaaaatt tatgatgaaa atggtgaatc
      481 tgctggttct ttgtctgttg ttaaaggtgg ttctggttat attaatattg gtccatctgc
      541 tccaggtcaa agagatagat tggttgaatt tagagaatct ggtggtaaaa ttcaaggtgg
      601 tccaccaggt tatagatata cttctgatca tgaagaagat caaagagata atagatatta
      661 taatactcat ggttatcatg ttggtgatgg tccagctgaa tatggtgatc atggtggtgg
      721 tcattggggt gatggttatt atggtccacc aggtgaattt gttaaaactt ctgaatatga
      781 agattgagct tagagaccaa gttccatccc aatggcgcgc cgagcttggc tcgagcatgg
      841 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      901 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      961 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1021 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1081 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1141 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1201 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1261 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1321 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1381 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1441 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1501 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1561 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1621 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1681 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1741 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1801 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1861 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1921 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1981 gagtaaactt ggtctgacag ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     2041 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     2101 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     2161 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     2221 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     2281 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     2341 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     2401 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     2461 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc agggatcgca
     2521 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     2581 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     2641 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     2701 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     2761 atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcatactc
     2821 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2881 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2941 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     3001 acgaggccct ttcgtc
//
