LOCUS       Exported                2932 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [306 nt] : (Uncut 5'[0] / Uncut 3'[306])
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..721
                     /label=BR32_00133701
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [306 nt] : (Uncut 5'[0] / Uncut 3'[306])"
     primer_bind     complement(756..772)
                     /label=M13Rv
     CDS             complement(1917..2732)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgggtccaa gatatattcc acatccagaa tgtgaagtta ctattatgaa
      481 accaggtact ccatctccaa gatctccata tactttgcca gaagaatatg aaagaggtta
      541 tggtccaggt gattctaaaa ctaaaattaa agttggtaaa tttttttgtg ttgctgattc
      601 tcattgtact ccaaattgtg gtagattgcc aaatgaaaat tggtatgctg atggtgctga
      661 agctccaaga tgtgaagatg gtttggcttc tatttataat tgagcttaga gaccaagttc
      721 catcccaatg gcgcgccgag cttggctcga gcatggtcat agctgtttcc tgtgtgaaat
      781 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      841 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      901 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      961 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1021 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1081 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1141 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1201 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1261 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1321 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1381 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1441 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1501 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1561 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1621 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1681 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1741 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1801 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1861 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttag
     1921 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     1981 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     2041 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     2101 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     2161 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     2221 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     2281 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     2341 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     2401 tctaatacct ggaatgctgt tttcccaggg atcgcagtgg tgagtaacca tgcatcatca
     2461 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     2521 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     2581 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     2641 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     2701 gagcaagacg tttcccgttg aatatggctc atactcttcc tttttcaata ttattgaagc
     2761 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2821 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2881 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
