LOCUS       Exported                2941 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.seq.xdna [2626 nt] : (EcoRV[413] / 
            EcoRV[413]) to Sequence Window #4 [315 nt] : (Uncut 3'[315] / Uncut 
            5'[0])
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             146..400
                     /label=LacZ
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..730
                     /label=br48_g1736
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [315 nt] : (Uncut 3'[315] / Uncut 5'[0])"
     primer_bind     765..781
                     /label=M13Rv
     CDS             1926..2741
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcattgt ctcctagtac ctggcaaacc accaaaaaaa taagtatcat
      481 aatctttaac aaattgtgga taatcatctg gaatcaattc agaatgttca gttggtcttt
      541 gaccacctgg aacatgacct ggatatggtt gccaagcagc agcagcagtt tgatgatatg
      601 gaactggttc ttcatattgt tgtggtggtg gaccacctgg acctctacca ccattaccac
      661 ctctaccacc ttgacctggt ctagatggtg gcattggttg tggagctggc aacattagag
      721 accaagttcc atcccaatgg cgcgccgagc ttggctcgag catggtcata gctgtttcct
      781 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      841 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      901 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      961 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1921 gacagttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     1981 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     2041 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     2101 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     2161 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     2221 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     2281 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     2341 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     2401 ggatattctt ctaatacctg gaatgctgtt ttcccaggga tcgcagtggt gagtaaccat
     2461 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     2521 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     2581 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     2641 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     2701 cgcggcctag agcaagacgt ttcccgttga atatggctca tactcttcct ttttcaatat
     2761 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2821 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2881 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     2941 c
//
