LOCUS       Exported                2923 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.seq.xdna [2626 nt] : (EcoRV[413] / 
            EcoRV[413]) to Sequence Window #4 [297 nt] : (Uncut 5'[0] / Uncut 
            3'[297])
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             146..400
                     /label=LacZ
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..712
                     /label=br48_g9741
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [297 nt] : (Uncut 5'[0] / Uncut 3'[297])"
     primer_bind     747..763
                     /label=M13Rv
     CDS             1908..2723
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgtcttttt ttgatggtga agatggttgt ttggttgaaa ttactgaaaa
      481 aactaaattt ttgggtttta ctactggtgg tggtaaatct ttgggtaatg attgtttgcc
      541 atttggttct caaagaccaa tttctgttac tggtactgat ggtaaaacta ttaatttgaa
      601 tgtttctgtt tctaaaaaat gtaaaactaa agttgaattg ggtccaccat tgccagataa
      661 tgctgttatt tctgcttttt ctgctgtttg ttgagcttag agaccaagtt ccatcccaat
      721 ggcgcgccga gcttggctcg agcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      781 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      841 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      901 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      961 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1021 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1081 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1141 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1201 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1261 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1321 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1381 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1441 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1501 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1561 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1621 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1681 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1741 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1801 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1861 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca
     1921 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     1981 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     2041 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     2101 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     2161 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     2221 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     2281 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     2341 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     2401 tggaatgctg ttttcccagg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     2461 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     2521 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     2581 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     2641 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac
     2701 gtttcccgtt gaatatggct catactcttc ctttttcaat attattgaag catttatcag
     2761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2821 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2881 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
