LOCUS       Exported                2890 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [264 nt] : (Uncut 5'[0] / Uncut 3'[264])
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..679
                     /label=br58_PWT4_69
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [264 nt] : (Uncut 5'[0] / Uncut 3'[264])"
     primer_bind     complement(714..730)
                     /label=M13Rv
     CDS             complement(1875..2690)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgaaaccac catctgattc tactgatgct tctaaaattc caacttataa
      481 aattaaacat ccaggtccaa gatatattat tactgatgat ggtagagttg ttgaagttga
      541 aagaaaagaa tctggtagat atcaattgat tgatggtgtt ttggttagag ttgaatatga
      601 aaaaaaaact ggtggttctg aaggttctaa aacttctact aatactagag gtccaaaatg
      661 agcttagaga ccaagttcca tcccaatggc gcgccgagct tggctcgagc atggtcatag
      721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1861 acttggtctg acagttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     1921 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     1981 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     2041 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     2101 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     2161 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     2221 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     2281 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     2341 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccagggat cgcagtggtg
     2401 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     2461 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     2521 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     2581 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     2641 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat actcttcctt
     2701 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2761 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2821 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2881 ccctttcgtc
//
