LOCUS       Exported                3034 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.seq.xdna [2626 nt] : (EcoRV[413] / 
            EcoRV[413]) to Sequence Window #4 [408 nt] : (Uncut 5'[0] / Uncut 
            3'[408])
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             146..400
                     /label=LacZ
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..823
                     /label=br58_g4698
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [408 nt] : (Uncut 5'[0] / Uncut 3'[408])"
     primer_bind     858..874
                     /label=M13Rv
     CDS             2019..2834
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgtctgatt tttggaaagt tgatttgtat ggtccaccac aaggtcattc
      481 taatgttaaa tctgaaattg ttgctactgt tgtttgtggt gatggtagaa aagctaattt
      541 gtcttatact cattcttgga gaggtgattc tactactaaa actattacta ttagatgtag
      601 aattactcca aaagttatta aaggtggtcc attgccacca tattatgaat tgaaagctga
      661 accaatttgg gctggtgatg aaagagctat tgctgctgct aaaagagaac aagaagaaag
      721 agttttggaa caatttgaat tgatgaattt gatgatggat gcttctgaag atccaaaagc
      781 tagaagattg tttgcttctc catgagctta gagaccaagt tccatcccaa tggcgcgccg
      841 agcttggctc gagcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      901 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      961 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1021 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1681 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1921 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1981 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt agaaaaactc atcgagcatc
     2041 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     2101 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     2161 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     2221 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     2281 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     2341 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     2401 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     2461 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     2521 gttttcccag ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     2581 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     2641 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     2701 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     2761 ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt
     2821 tgaatatggc tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2881 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2941 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     3001 tataaaaata ggcgtatcac gaggcccttt cgtc
//
