LOCUS       Exported                3043 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [417 nt] : (Uncut 5'[0] / Uncut 3'[417])
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..832
                     /label=CD156_00118721
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [417 nt] : (Uncut 5'[0] / Uncut 3'[417])"
     primer_bind     complement(867..883)
                     /label=M13Rv
     CDS             complement(2028..2843)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atggaagttt tttctgctgg tgttgctgct actggtcaat tgtgttgtac
      481 taatggtgtt ccagatgatt ctccaacttg taaaaaattg ggtttgaact cttatgctgt
      541 tattccaact ttttctttta cttctgaaac tccagaaaaa aataaagaat tgactttttt
      601 ttctatgcaa tgtgaatctg ttagaagaaa tgatgaaaaa gctcatagag aagatccagg
      661 tagaaaaaat ggttgtgata ataaattgtt ggaaggtttg tttccagttg gtagagatgt
      721 taaagctttt gttccaagat ctactaatac tgttgaattg ggtccaactt ctaaagttaa
      781 tactatgttt gcttttattg gttgtgctgc ttgagcttag agaccaagtt ccatcccaat
      841 ggcgcgccga gcttggctcg agcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      901 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      961 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1021 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1081 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1141 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1201 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1261 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1321 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1381 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1441 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1501 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1561 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1621 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1681 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1741 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1801 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1861 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1921 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1981 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca
     2041 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     2101 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     2161 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     2221 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     2281 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     2341 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     2401 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     2461 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     2521 tggaatgctg ttttcccagg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     2581 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     2641 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     2701 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     2761 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac
     2821 gtttcccgtt gaatatggct catactcttc ctttttcaat attattgaag catttatcag
     2881 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2941 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3001 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
