LOCUS       Exported                3217 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [591 nt] : (Uncut 3'[591] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..1006)
                     /label=CD156_00123591
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [591 nt] : (Uncut 3'[591] / Uncut 5'[0])"
     primer_bind     complement(1041..1057)
                     /label=M13Rv
     CDS             complement(2202..3017)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcattta tgaacaccag tccattcata accaaaatct tctggcaaat
      481 ccaaaataat atgagccaat tcatgcataa tagtagtatc ttgattagta gcagtaattt
      541 cagaagaaga ttctggttga ttaaaaaaat ttggacaaat aacaatttgt tctttagcaa
      601 ttctagcata acccaaaaca tttttataac cttgattttc tttacattta ccagtttcat
      661 cttcacaagt aatataaact tcatcttctt ttctagaaca ttcttcatac aattttttaa
      721 aattttgttc aaccaaatct tgaatatcag tagaatcagt tttaaaaata aattcaaaca
      781 attcttcatc agatttaaca gcatgaaaag catgttgagc caaaacagca caagattcca
      841 aagcacctct aatttcagaa gcttcagaag aagaacattg agaacctttt tgaatataag
      901 ctcttttatt caaagcacca tgaatatcat tagaaaattc tttataatta ttaattggtt
      961 gcaaaaaagt accagtatta ataaaagcca ttagagacca agttccatcc caatggcgcg
     1021 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1081 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1141 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1201 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1261 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1381 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1441 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1561 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1621 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1681 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1741 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1801 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1861 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1921 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1981 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2041 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2101 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2161 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc
     2221 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2281 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2341 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2401 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2461 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2521 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2581 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2641 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2701 gctgttttcc cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2761 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2821 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2881 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2941 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     3001 cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta tcagggttat
     3061 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3121 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3181 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
