LOCUS       Exported                2881 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [255 nt] : (Uncut 5'[0] / Uncut 3'[255])
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..670
                     /label=CD156_00133411
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [255 nt] : (Uncut 5'[0] / Uncut 3'[255])"
     primer_bind     complement(705..721)
                     /label=M13Rv
     CDS             complement(1866..2681)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgtttccaa tgaaagctaa ttgtggtcca actacttcta gagaattgca
      481 aaatggtatt acttctggta ctaaagctgc tgttaatcat ggttctaatt tgcatactag
      541 agaagaagaa ccagttgaac aaaatttgcc atgtggtaaa tgtggtactt atactactaa
      601 tgatggtact ttgtatttta gacatttttc tgattgtgat ggtaaatctt gagcttagag
      661 accaagttcc atcccaatgg cgcgccgagc ttggctcgag catggtcata gctgtttcct
      721 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      781 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      841 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      901 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      961 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1021 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1081 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1141 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1201 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1261 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1321 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1381 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1441 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1501 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1561 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1621 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1681 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1741 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1801 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1861 gacagttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     1921 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     1981 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     2041 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     2101 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     2161 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     2221 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     2281 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     2341 ggatattctt ctaatacctg gaatgctgtt ttcccaggga tcgcagtggt gagtaaccat
     2401 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     2461 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     2521 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     2581 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     2641 cgcggcctag agcaagacgt ttcccgttga atatggctca tactcttcct ttttcaatat
     2701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2761 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2821 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     2881 c
//
