LOCUS       Exported                2797 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [171 nt] : (Uncut 3'[171] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..586)
                     /label=CD156_00138051
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [171 nt] : (Uncut 3'[171] / Uncut 5'[0])"
     primer_bind     complement(621..637)
                     /label=M13Rv
     CDS             complement(1782..2597)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaaccc catctcaatc tagaaaaatg atgagctgga gttcttctca
      481 aaaacaaaga accatgagca attggaactt tagaagaata caaagtttga tcaatacaag
      541 caatccaatg ttcacattta cctctagcca ttagagacca agttccatcc caatggcgcg
      601 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      661 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      721 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      781 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      841 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      901 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      961 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1021 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1081 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1141 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1201 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1261 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1321 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1381 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1441 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1501 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1561 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1621 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1681 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1741 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc
     1801 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     1861 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     1921 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     1981 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2041 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2101 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2161 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2221 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2281 gctgttttcc cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2341 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2401 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2461 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2521 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     2581 cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta tcagggttat
     2641 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2701 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2761 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
