LOCUS       Exported                3007 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [381 nt] : (Uncut 5'[0] / Uncut 3'[381])
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..796
                     /label=FR13_00020841
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [381 nt] : (Uncut 5'[0] / Uncut 3'[381])"
     primer_bind     complement(831..847)
                     /label=M13Rv
     CDS             complement(1992..2807)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgattaatg aagattgtat tgtttctttg gttccagatg gtggttcttt
      481 tgctagaaaa actttgagat cttctccagg tcaacaacat gatttgcaat ggggtaaata
      541 tgcttttact attagagttg aaaatggttg tgctggtaaa aaaactgctg gtactattcc
      601 accagctcat tttgtttctg ttggtagagc tgcttctttg aaaccaactt ctattttgag
      661 agatggtgat ccaagaccac caactgctgt ttatccacca agacaaccac caagaactga
      721 aggtagaaga actagatatg gttcttctgg tggtgctgtt actgttggtg ctagatgagc
      781 ttagagacca agttccatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg
      841 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      901 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      961 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1021 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1081 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1141 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1201 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1261 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1321 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1381 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1441 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1501 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1561 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1621 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1681 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1741 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1801 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1861 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1921 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1981 tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     2041 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     2101 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     2161 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     2221 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     2281 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     2341 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     2401 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2461 gaatcaggat attcttctaa tacctggaat gctgttttcc cagggatcgc agtggtgagt
     2521 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2581 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2641 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2701 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2761 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt
     2821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2881 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2941 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     3001 tttcgtc
//
