LOCUS       Exported                2889 bp ds-DNA     circular SYN 11-DEC-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan [2626 nt] : (EcoRV[413] / EcoRV[413]) to 
            Untitled Sequence #1 [263 nt] : (Uncut 5'[0] / Uncut 3'[263])
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..678
                     /label=FR13_00057131
                     /note="note=""Fragment extracted from Untitled Sequence #1 
                     [263 nt] : (Uncut 5'[0] / Uncut 3'[263])"
     primer_bind     complement(713..729)
                     /label=M13Rv
     CDS             complement(1874..2689)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatttggt
      421 ctctaatgca tggttggcaa tggtgtattg ttactgtttt ttatcaaggt agatctactg
      481 gtgaaacttt tccaactgct aaaaaagttg gtagaaaata tgaatttcca ggtgaaggta
      541 aaagatatgc tccatataaa tttgtttgtg atgatcaatg taaatgtgtt tctgctggta
      601 gaccattttt gactggtatg agattggaag gtcaaggtca tgttccaaaa gctaaacatg
      661 aatgagctta gagaccaaat cccaatggcg cgccgagctt ggctcgagca tggtcatagc
      721 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      781 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      841 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      901 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      961 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1021 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1081 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1141 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1201 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1261 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1321 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1381 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1441 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1501 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1561 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1621 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1681 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1741 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1801 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1861 cttggtctga cagttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1921 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1981 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     2041 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     2101 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
     2161 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     2221 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     2281 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     2341 ctgaatcagg atattcttct aatacctgga atgctgtttt cccagggatc gcagtggtga
     2401 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     2461 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     2521 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     2581 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     2641 aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt
     2701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2821 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2881 cctttcgtc
//
