LOCUS       Exported                2866 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [240 nt] : (Uncut 3'[240] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..655)
                     /label=FR13_00066391
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [240 nt] : (Uncut 3'[240] / Uncut 5'[0])"
     primer_bind     complement(690..706)
                     /label=M13Rv
     CDS             complement(1851..2666)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcattca caacccaaaa cttcttcaat ttcaccttta atagtaactt
      481 tttcagcaac caaatcagca ccaaaaataa ttggagtaca atcattttca aattcaacat
      541 gaatatcacc atgtttcata tattcagtat tcaaacaaga accagattca ataatttcac
      601 cttcagagtt caaaacagtc catttacaca atttagccat tagagaccaa gttccatccc
      661 aatggcgcgc cgagcttggc tcgagcatgg tcatagctgt ttcctgtgtg aaattgttat
      721 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      781 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      841 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      901 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      961 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1021 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1081 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1141 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1201 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1261 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1321 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1381 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1441 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1501 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     1561 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1621 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1681 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1741 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1801 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttagaaaaac
     1861 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     1921 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     1981 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     2041 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     2101 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
     2161 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     2221 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     2281 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     2341 acctggaatg ctgttttccc agggatcgca gtggtgagta accatgcatc atcaggagta
     2401 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     2461 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     2521 gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga
     2581 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa
     2641 gacgtttccc gttgaatatg gctcatactc ttcctttttc aatattattg aagcatttat
     2701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2761 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2821 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
