LOCUS       Exported                2935 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [309 nt] : (Uncut 3'[309] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..724)
                     /label=FR13_00069491
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [309 nt] : (Uncut 3'[309] / Uncut 5'[0])"
     primer_bind     complement(759..775)
                     /label=M13Rv
     CDS             complement(1920..2735)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaagca gcacaaccaa caaaacctgg caaaccacca gaagtacaat
      481 caccattaat tggatcaaca aaagtaacat ctctaccaac tggaaaagaa gattgcaatg
      541 gatcacaacc agattgacct ttaccagatc ttttagtatc agatctaaaa tgaccacaac
      601 aataagaatt ttgattaaaa gatttacaaa ttttatctgg ttgtggagta cctctatcac
      661 aacaaatttt accagtttga gaaccttgaa aatctggagc aacagccatt agagaccaag
      721 ttccatccca atggcgcgcc gagcttggct cgagcatggt catagctgtt tcctgtgtga
      781 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      841 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      901 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
      961 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1021 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1081 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1141 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1201 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1261 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1321 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1381 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1441 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1501 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1561 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1621 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1681 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1741 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1801 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1861 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1921 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     1981 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     2041 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     2101 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     2161 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     2221 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     2281 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     2341 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     2401 tcttctaata cctggaatgc tgttttccca gggatcgcag tggtgagtaa ccatgcatca
     2461 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     2521 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     2581 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     2641 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     2701 ctagagcaag acgtttcccg ttgaatatgg ctcatactct tcctttttca atattattga
     2761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2881 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
