LOCUS       Exported                2956 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [330 nt] : (Uncut 5'[0] / Uncut 3'[330])
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..745
                     /label=FR13_00087831
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [330 nt] : (Uncut 5'[0] / Uncut 3'[330])"
     primer_bind     complement(780..796)
                     /label=M13Rv
     CDS             complement(1941..2756)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgaataatt ttactgttgg tagaggttct actggtggta gatgttgtga
      481 tcatggtgtt gctgatccat ctagaacttg ttctaaaatg aaattgaact cttattcttg
      541 tattgatttt agatctgatg ctaaagctgg tgattctgtt aatgatgttg gtggtggttg
      601 tgatccagtt gaattgagaa attggccaat tggtagagat gttaaagctt ttgttccagg
      661 ttctgttgct actcatcaaa cttctgattt tgatttggaa gttggtttta ttggttgtgc
      721 tgaatgagct tagagaccaa gttccatccc aatggcgcgc cgagcttggc tcgagcatgg
      781 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      841 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      901 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      961 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1021 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1081 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1141 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1201 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1261 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1321 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1381 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1441 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1501 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1561 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1621 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1681 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1741 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1801 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1861 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     1921 gagtaaactt ggtctgacag ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     1981 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     2041 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     2101 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     2161 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc
     2221 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     2281 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     2341 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     2401 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc agggatcgca
     2461 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     2521 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     2581 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     2641 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     2701 atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcatactc
     2761 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2821 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2881 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2941 acgaggccct ttcgtc
//
