LOCUS       Exported                2911 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [285 nt] : (Uncut 3'[285] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..700)
                     /label=FR13_00093031
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [285 nt] : (Uncut 3'[285] / Uncut 5'[0])"
     primer_bind     complement(735..751)
                     /label=M13Rv
     CDS             complement(1896..2711)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaagat tcaccagcaa tagcagtaac ttttttataa acatgcaaac
      481 cccaaccttt tggaacagat tttttaacat catcagtatt agttggatta caattagcat
      541 caacagaaat aacaacttca gtagaaaatt cagttggttg aaaagcaata gaaccattag
      601 ctggcaaagc agaaccataa taagtttctt gaccaccatt agcatcatat ttcaaaatag
      661 aagcagaaca accatcttcc atcattagag accaagttcc atcccaatgg cgcgccgagc
      721 ttggctcgag catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      781 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      841 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1081 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1141 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1201 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1261 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1321 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1381 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1441 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1501 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1561 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1621 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1681 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1741 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1801 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1861 atctaaagta tatatgagta aacttggtct gacagttaga aaaactcatc gagcatcaaa
     1921 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     1981 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     2041 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     2101 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt
     2161 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     2221 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     2281 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     2341 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     2401 ttcccaggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     2461 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     2521 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     2581 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     2641 tataaatcag catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga
     2701 atatggctca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2761 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2821 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2881 aaaaataggc gtatcacgag gccctttcgt c
//
