LOCUS       Exported                2872 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [246 nt] : (Uncut 5'[0] / Uncut 3'[246])
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..661
                     /label=FR13_00094721
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [246 nt] : (Uncut 5'[0] / Uncut 3'[246])"
     primer_bind     complement(696..712)
                     /label=M13Rv
     CDS             complement(1857..2672)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atggatgatg ttaaaaaaga ttgttattat actgtttgga cttctaatgg
      481 taattcttgg ttgagatcta ttagagataa aacttggtct gatcatactt tgggtattca
      541 tggtgcttct gtttattttg atgaagattg ttatccacat tatcattcta aagaaatttt
      601 ggttagaaaa ggttgtcatg aagaaactac tattccaaaa tgagcttaga gaccaagttc
      661 catcccaatg gcgcgccgag cttggctcga gcatggtcat agctgtttcc tgtgtgaaat
      721 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      781 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      841 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
      901 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      961 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1021 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1081 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1141 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1261 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1501 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1561 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1621 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1681 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1741 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1801 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttag
     1861 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     1921 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     1981 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     2041 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     2101 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     2161 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     2221 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     2281 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     2341 tctaatacct ggaatgctgt tttcccaggg atcgcagtgg tgagtaacca tgcatcatca
     2401 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     2461 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     2521 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     2581 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     2641 gagcaagacg tttcccgttg aatatggctc atactcttcc tttttcaata ttattgaagc
     2701 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2761 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2821 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
