LOCUS       Exported                2887 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [261 nt] : (Uncut 3'[261] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..676)
                     /label=FR13_00104931
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [261 nt] : (Uncut 3'[261] / Uncut 5'[0])"
     primer_bind     complement(711..727)
                     /label=M13Rv
     CDS             complement(1872..2687)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaaact ttaatcatat aacctttaac agaccaattt tttggcaaaa
      481 caacatcttt agtttcacat ctagtattac atttaatagt atgaccttta ataacaaaat
      541 ctttatccca attacctcta gccataccaa caaatctagc attattatca aacatttcaa
      601 ctttacacaa ttgtggcaaa acagcagctg gtttagatgg agctggagta gctggagcca
      661 ttagagacca agttccatcc caatggcgcg ccgagcttgg ctcgagcatg gtcatagctg
      721 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
      781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
      841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      901 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1081 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1741 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1861 tggtctgaca gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1921 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1981 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     2041 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     2101 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     2161 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     2221 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     2281 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2341 gaatcaggat attcttctaa tacctggaat gctgttttcc cagggatcgc agtggtgagt
     2401 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2461 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2521 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2581 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2641 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt
     2701 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2761 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2821 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     2881 tttcgtc
//
