LOCUS       Exported                3097 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [471 nt] : (Uncut 5'[0] / Uncut 3'[471])
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..886
                     /label=FR13_00108711
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [471 nt] : (Uncut 5'[0] / Uncut 3'[471])"
     primer_bind     complement(921..937)
                     /label=M13Rv
     CDS             complement(2082..2897)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgggtgatg aatctattgg ttctacttct ccaaggagac caggttctcc
      481 aactgatcca ggttctttgt tggctgctgg tggttctcca ggttctagat ctgctccagc
      541 tactgatggt gctttgccac caagagatca aggtatgatt ggttttggtc caattccatc
      601 tgctagaaat agaaaaaaat ttccacaacc aaatattgtt ccaggtccag atggtactgc
      661 tgctcatcca gaaggtccaa tgtgtattat tactttggtt agaccatttg gtagatctga
      721 atctttgcca ttgagagctg gtgttgaagg tattccagat ggttggtctt ggaaatggag
      781 aaaagctcca tatgctcaag ataaaaaaaa atctagagtt tttggtggtt tgggtagatt
      841 tggtagaact tctcaaagaa ctggttgagc ttagagacca agttccatcc caatggcgcg
      901 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      961 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     1021 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1081 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1141 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1201 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1261 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1321 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1381 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1441 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1501 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1561 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1621 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1681 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1741 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1801 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1861 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1921 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1981 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2041 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc
     2101 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2161 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2221 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2281 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2341 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2401 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2461 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2521 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2581 gctgttttcc cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2641 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2701 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2761 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2821 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     2881 cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta tcagggttat
     2941 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3001 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3061 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
