LOCUS       Exported                2953 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [327 nt] : (Uncut 5'[0] / Uncut 3'[327])
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..742
                     /label=FR13_00109161
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [327 nt] : (Uncut 5'[0] / Uncut 3'[327])"
     primer_bind     complement(777..793)
                     /label=M13Rv
     CDS             complement(1938..2753)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgaaaggtc caaaaactat tgaagttcaa ccagattttc aaggtccaca
      481 aactggtggt atttgttgtg atgctggtac taattctgat actgataaat tttgttctgg
      541 taataatttg aatgcttttt gttgtggtcc atttagatct gatagaaaag gtggtaaagg
      601 tgttcaaggt ggttgtgatc catttccaga ttttccaact ggtagaaatg ttgttacttt
      661 tccaccaggt aatcaacaat gtgtttcttc tggtggtcat gctggtttta ttggttgtgc
      721 ttgagcttag agaccaagtt ccatcccaat ggcgcgccga gcttggctcg agcatggtca
      781 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      841 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      901 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      961 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1021 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1081 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1141 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1201 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1261 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1321 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1381 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1441 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1501 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1561 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1621 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1681 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1741 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1801 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1861 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1921 taaacttggt ctgacagtta gaaaaactca tcgagcatca aatgaaactg caatttattc
     1981 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     2041 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     2101 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     2161 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     2221 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     2281 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     2341 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     2401 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccagg gatcgcagtg
     2461 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     2521 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     2581 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     2641 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     2701 ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct catactcttc
     2761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtc
//
