LOCUS       Exported                2977 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [351 nt] : (Uncut 5'[0] / Uncut 3'[351])
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..766
                     /label=FR13_00122001
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [351 nt] : (Uncut 5'[0] / Uncut 3'[351])"
     primer_bind     complement(801..817)
                     /label=M13Rv
     CDS             complement(1962..2777)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atggttgctt tgccagctga tccagctcca gctccagctc cagttagacc
      481 agtttttggt tgttttgtta gaatgtctat tgttactact gataaagatt ctacttctat
      541 taattctggt gatttgattc caactactaa tccaggtcaa gttgatttta aagatggttc
      601 tgatactatt actgttaaat tggataaaaa ttgtcaacca gttggtaata ttaaacatag
      661 aaaagaagtt caatttaatg ctttgcaaaa attgactaaa aatggtggtc catttactga
      721 tccagatcaa ccatttatta ctttttgagc ttagagacca agttccatcc caatggcgcg
      781 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      841 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      901 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
      961 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1021 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1081 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1141 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1201 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1261 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1321 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1381 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1441 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1501 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1561 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1621 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1681 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc
     1981 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2041 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2101 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2161 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2221 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2281 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2341 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2401 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2461 gctgttttcc cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2521 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2581 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2641 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2701 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     2761 cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta tcagggttat
     2821 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2881 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2941 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
