LOCUS       Exported                3025 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [399 nt] : (Uncut 5'[0] / Uncut 3'[399])
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..814
                     /label=FR13_00128431
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [399 nt] : (Uncut 5'[0] / Uncut 3'[399])"
     primer_bind     complement(849..865)
                     /label=M13Rv
     CDS             complement(2010..2825)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgggtagag gttcttattt gactgttaaa ccatctgaat tggataaaca
      481 tattgctcaa ttggaagaat atttgagaaa tccaccatgt aaagttacta tttatgctcc
      541 accaccaaaa aaaggtagaa ctcaattgag aactgaaatg ggttttgctt ctgctggtaa
      601 aaatgaagat gttccagtta aaattgcttc tactggtcat acttatcaat gtagaactga
      661 tgctacttgt gaagaaccag tttgtcatgg tttgccaaat ggttggactt atgatggtgt
      721 ttatatgcat acttttactg aattgtcttt ggaacaattg aaagctcaaa aagctgctca
      781 aacttctaga cattgagctt agagaccaag ttccatccca atggcgcgcc gagcttggct
      841 cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      901 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      961 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1021 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1081 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1141 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1201 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1261 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1321 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1381 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1441 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1501 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1561 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1621 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1681 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1741 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1801 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1861 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1921 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1981 agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat caaatgaaac
     2041 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     2101 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     2161 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     2221 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     2281 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2341 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2401 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     2461 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccca
     2521 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2581 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     2641 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     2701 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     2761 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     2821 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2941 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     3001 aggcgtatca cgaggccctt tcgtc
//
