LOCUS       Exported                2992 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [366 nt] : (Uncut 3'[366] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..781)
                     /label=FR13_00144271
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [366 nt] : (Uncut 3'[366] / Uncut 5'[0])"
     primer_bind     complement(816..832)
                     /label=M13Rv
     CDS             complement(1977..2792)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcattta gcacaaccaa taaaagcatt ataaacattt ttttgagaat
      481 cagttctttt acaagtacca ccttgaacaa aagaagtaac agttctacca acattaaaaa
      541 tttcaattct tggtggatca caaccacctc tattatcatt tctagcttga gaacattgtg
      601 gagatggatg attaattttt ttacctttag atctagcagt aataatttta acacaagaaa
      661 cacaataaga gttcaaaccc aaacctttac aagattcaga agaatctgga gtaccttgat
      721 cacaacacaa ttcaccagta cctggattaa tattaatttc aaccattaga gaccaagttc
      781 catcccaatg gcgcgccgag cttggctcga gcatggtcat agctgtttcc tgtgtgaaat
      841 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
      901 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
      961 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     1801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     1861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttag
     1981 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     2041 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     2101 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     2161 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     2221 tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca
     2281 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     2341 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     2401 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     2461 tctaatacct ggaatgctgt tttcccaggg atcgcagtgg tgagtaacca tgcatcatca
     2521 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     2581 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     2641 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     2701 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta
     2761 gagcaagacg tttcccgttg aatatggctc atactcttcc tttttcaata ttattgaagc
     2821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     2941 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
