LOCUS       Exported                2995 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [369 nt] : (Uncut 3'[369] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..784)
                     /label=GY11_00067931
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [369 nt] : (Uncut 3'[369] / Uncut 5'[0])"
     primer_bind     complement(819..835)
                     /label=M13Rv
     CDS             complement(1980..2795)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaagca acaactttat aatatttaat aatagtacca tcatcatctt
      481 tagaatcagc atgaaattga catctatttt tatcaattgg attacattct ttatcccatt
      541 tacaaccagc aaattcatat ggaacaaatt tccaagaata tttagatcta ccaactcttt
      601 caacttgagt accaatcaaa gtatcaccat caaacaacaa tttataaaaa ataccaaatt
      661 cacaacctgg aactctttta ttaaaagcat tcaatctaaa atcagtaatt ggtctaactt
      721 caatcaattt cattggaact tctggtggtg gtttttttgg tggacccatt agagaccaag
      781 ttccatccca atggcgcgcc gagcttggct cgagcatggt catagctgtt tcctgtgtga
      841 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      901 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
      961 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1021 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1081 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1141 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1201 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1681 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1861 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1921 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     1981 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     2041 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     2101 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     2161 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     2221 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     2281 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     2341 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     2401 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     2461 tcttctaata cctggaatgc tgttttccca gggatcgcag tggtgagtaa ccatgcatca
     2521 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     2581 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     2641 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     2701 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     2761 ctagagcaag acgtttcccg ttgaatatgg ctcatactct tcctttttca atattattga
     2821 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2881 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2941 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
