LOCUS       Exported                3055 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [429 nt] : (Uncut 5'[0] / Uncut 3'[429])
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..844
                     /label=GY11_00072321
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [429 nt] : (Uncut 5'[0] / Uncut 3'[429])"
     primer_bind     complement(879..895)
                     /label=M13Rv
     CDS             complement(2040..2855)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atggcttctc catctactcc agcttcttat tctgctgaag ctgctgctgc
      481 tgctgctttg ggtccattga ctagaattga taaaccattt actcaatgta gaattactta
      541 tactgctcca aataaaaaac cagttgttaa taaattgggt catggtggta ctgctaaatt
      601 gtctgttgat ggtgttactt atactgctgt tgctgatgct aaatgtaaat tggttgttgt
      661 tggttctcca tggccagctg gtcattttac tgttggtcaa gttgttaaag gtaaagttgg
      721 tgttccacca agaatggaaa tttgggttga tgaagaagat attgttccaa aaatgtctag
      781 attgggtttg ggtaggagac caggtagagc tagacatggt caatgagctt agagaccaag
      841 ttccatccca atggcgcgcc gagcttggct cgagcatggt catagctgtt tcctgtgtga
      901 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
      961 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1021 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1081 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1141 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1201 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1261 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1321 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1381 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1441 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1501 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1561 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1621 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1681 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1741 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1801 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1861 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1921 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1981 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2041 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     2101 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     2161 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     2221 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     2281 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     2341 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     2401 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     2461 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     2521 tcttctaata cctggaatgc tgttttccca gggatcgcag tggtgagtaa ccatgcatca
     2581 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     2641 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     2701 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     2761 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     2821 ctagagcaag acgtttcccg ttgaatatgg ctcatactct tcctttttca atattattga
     2881 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2941 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     3001 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
