LOCUS       Exported                2893 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [267 nt] : (Uncut 5'[0] / Uncut 3'[267])
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..682
                     /label=GY11_00074021
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [267 nt] : (Uncut 5'[0] / Uncut 3'[267])"
     primer_bind     complement(717..733)
                     /label=M13Rv
     CDS             complement(1878..2693)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atgaaaggtt gtccatattc tgttagaaga tatgataaag aagttaaatg
      481 ggatattgtt gaagctggta aatctcatat tagagttatt gatggttatc cagctactat
      541 ttctgctgat caagattgta aattgaaaat taaaggtttg gctagattga aatatactat
      601 tgctccaggt gattcttcta ctattaaacc aaaaagaggt tgtactgctc caagagttac
      661 ttgagcttag agaccaagtt ccatcccaat ggcgcgccga gcttggctcg agcatggtca
      721 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      781 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      841 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      901 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      961 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1021 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1321 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1381 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1441 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1501 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1561 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1621 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1681 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1741 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1861 taaacttggt ctgacagtta gaaaaactca tcgagcatca aatgaaactg caatttattc
     1921 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     1981 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     2041 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     2101 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     2161 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     2221 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     2281 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     2341 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccagg gatcgcagtg
     2401 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     2461 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     2521 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     2581 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     2641 ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct catactcttc
     2701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2821 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2881 aggccctttc gtc
//
