LOCUS       Exported                3037 bp ds-DNA     circular SYN 14-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 14, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [411 nt] : (Uncut 3'[411] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..826)
                     /label=GY11_00148071
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [411 nt] : (Uncut 3'[411] / Uncut 5'[0])"
     primer_bind     complement(861..877)
                     /label=M13Rv
     CDS             complement(2022..2837)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaagct tgagctttag taccagtttt agtaccagtt ttagaaccag
      481 ttttagtaga tggtttagta gatggtttag taccagcagc agtatctggt ttagtagaag
      541 attttggtgg tttaccttct ttatcaacag aaccttcaat tttaaaacca gttggcaaag
      601 ctggagaaac agaagtaaat tcacatttag aattaacttt agcagtaaaa acaccagcac
      661 cagaagtatc agacaaaata gtagtaccag atggtggcaa caacatagca tcaacttctc
      721 ttttattacc attagtcaaa gccaatttac aagaagttgg aactggtttt ttaaaaatat
      781 caaaaatacc tggaactgga gttggagcca ttagagacca agttccatcc caatggcgcg
      841 ccgagcttgg ctcgagcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
      901 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      961 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1021 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1081 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1141 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1201 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1261 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1321 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1381 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1441 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1501 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1561 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1621 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1681 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1741 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1801 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1861 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1921 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1981 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttagaaaaa ctcatcgagc
     2041 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2101 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2161 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2221 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2281 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2341 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2401 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2461 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2521 gctgttttcc cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2581 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2641 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2701 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2761 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     2821 cgttgaatat ggctcatact cttccttttt caatattatt gaagcattta tcagggttat
     2881 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2941 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     3001 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
