LOCUS       Exported                3280 bp ds-DNA     circular SYN 06-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.seq.xdna [2626 nt] : (EcoRV[413] / 
            EcoRV[413]) to Sequence Window #4 [654 nt] : (Uncut 3'[654] / Uncut 
            5'[0])
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..1069
                     /label=ina168_g11067
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [654 nt] : (Uncut 3'[654] / Uncut 5'[0])"
     primer_bind     complement(1104..1120)
                     /label=M13Rv
     CDS             2265..3080
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaacaa tatttatatc tagcacattg agcaaaaata gcataagaat
      481 ctggattttt aatagattca gtattatgaa taccatccca ttcacaacca taatctttcc
      541 attctttcaa aataatatga accatttcat gcaaaataat agtatcttga ttaccagcag
      601 taatttctct agaattaact ggattattaa aaaatggtgg acaaataaca atttcttttt
      661 cattaatcaa agcataagcc aaattatgaa cttcttctct aacacaagta taaacatttt
      721 tatcatgaca agtcaaagaa atttcatcag catctctatt acattcttta taaatttcat
      781 taaaattatt ttgaacccaa ttttgaatat cagtagaatc agttttaaaa atcaatttaa
      841 acaatctatt attagattta acagcatgat aaccccaaga agccaattca gcacaagatt
      901 tcaaagcagc tctaatttca gaagcttgag aatcagaaca ttgagaatat ctttcaatat
      961 aagctcttct tttcaaatca ccatgaatat gatttggaat tggattataa tcataaactg
     1021 gatgagaaaa agtaccaata ttagtaaaag ccattagaga ccaagttcca tcccaatggc
     1081 gcgccgagct tggctcgagc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa aaactcatcg
     2281 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     2341 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     2401 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     2461 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     2521 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     2581 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     2641 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     2701 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     2761 aatgctgttt tcccagggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     2821 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     2881 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     2941 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     3001 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     3061 tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3181 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3241 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
