LOCUS       Exported                3001 bp ds-DNA     circular SYN 06-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2018 from SnapGene Viewer 4.1.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.seq.xdna [2626 nt] : (EcoRV[413] / 
            EcoRV[413]) to Sequence Window #4 [375 nt] : (Uncut 5'[0] / Uncut 
            3'[375])
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..790
                     /label=ina168_g4671
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [375 nt] : (Uncut 5'[0] / Uncut 3'[375])"
     primer_bind     complement(825..841)
                     /label=M13Rv
     CDS             1986..2801
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta atggctatgc catctgatac tactggtaaa ccatctggta ctactgttga
      481 tccatctaaa ccagattatt ctgctccaag atattggatt gataaatgta ttgttcaatt
      541 gttggatgct actactgaag aaattgttat ggaaagattt tcttctggtt ttggtatgtg
      601 ggattctgtt tcttatttgg gtcatactta tcaatttaga ttggatcaag attgtaaaac
      661 tatttggggt gattctagag gttctattca tccatctgtt aaatttagaa aagttagagc
      721 tagagttaat atgcatacta gaaaattaga tccattgtat gttgttgatt gagcttagag
      781 accaagttcc atcccaatgg cgcgccgagc ttggctcgag catggtcata gctgtttcct
      841 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      901 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      961 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1021 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1081 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1141 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1201 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1261 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1321 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1381 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1441 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1501 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1561 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1621 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1681 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1801 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1861 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1921 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1981 gacagttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     2041 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     2101 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     2161 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     2221 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     2281 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     2341 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     2401 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     2461 ggatattctt ctaatacctg gaatgctgtt ttcccaggga tcgcagtggt gagtaaccat
     2521 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     2581 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     2641 agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc
     2701 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     2761 cgcggcctag agcaagacgt ttcccgttga atatggctca tactcttcct ttttcaatat
     2821 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2881 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     2941 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3001 c
//
