LOCUS       Exported                2845 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan.xdna [2626 nt] : (EcoRV[413] / EcoRV[413]) 
            to Sequence Window #4 [219 nt] : (Uncut 3'[219] / Uncut 5'[0])
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             complement(416..634)
                     /label=US71_00048451
                     /note="note=""Fragment extracted from Sequence Window #4 
                     [219 nt] : (Uncut 3'[219] / Uncut 5'[0])"
     primer_bind     complement(669..685)
                     /label=M13Rv
     CDS             complement(1830..2645)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggaac
      421 ttggtctcta agctcaaaca cattgacctc tagctctatg ttcaaaaaaa tcattacctt
      481 taacaaattc tttttcacat ttaccacatc tataatgtgg agttggagta tcttcttctc
      541 tagcttgcaa tttagcacct ctagtagcag caacatctct acctggagca ccagcacatc
      601 tagaagcagt tggcaacatt agagaccaag ttccatccca atggcgcgcc gagcttggct
      661 cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      721 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      781 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      841 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      901 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      961 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1021 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1081 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1141 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1201 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1261 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1321 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1381 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1441 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1501 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1561 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1621 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1681 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1801 agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat caaatgaaac
     1861 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1921 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1981 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     2041 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     2101 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2161 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2221 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     2281 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccca
     2341 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2401 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     2461 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     2521 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     2581 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
     2641 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2701 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2761 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2821 aggcgtatca cgaggccctt tcgtc
//
