LOCUS       Exported                3033 bp ds-DNA     circular SYN 11-DEC-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 17, 2018 from SnapGene Viewer 4.2.9
            http://www.snapgene.com
COMMENT     Ligation of : pUC57-Kan [2626 nt] : (EcoRV[413] / EcoRV[413]) to 
            Untitled Sequence #1 [407 nt] : (Uncut 5'[0] / Uncut 3'[407])
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     352..375
                     /label=M13Fw(-47)
     primer_bind     378..395
                     /label=M13Fw(-21)
     CDS             416..822
                     /label=US71_00116921
                     /note="note=""Fragment extracted from Untitled Sequence #1 
                     [407 nt] : (Uncut 5'[0] / Uncut 3'[407])"
     primer_bind     complement(857..873)
                     /label=M13Rv
     CDS             complement(2018..2833)
                     /label=KanR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatttggt
      421 ctctaatgtt gccagctact actcaaccaa ataatatttt ggctggtttg ccaactcaac
      481 cagctaatcc agctccaaaa attcaatatg atttgtgtaa agttttgttg tggaaagata
      541 aaaaagaaca atttggtaat aaaggttctt cttctggttt tggttctcca ggtaatactg
      601 ttagagtttg gatgcatcca ggtttggctg ctgtttttca attgtctgaa gaaggttgtg
      661 aaggtaaatt tattggtggt gaattggatc aaggtaaaac tagaactcca ttgtctaaag
      721 gtgatccaag attgatggaa gctttgaaag attataaagg tgctgttttt ccagaaccac
      781 atggtgatgc tactatgatt aaatggtgag cttagagacc aaatcccaat ggcgcgccga
      841 gcttggctcg agcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1141 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1201 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1261 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1321 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1381 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1441 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1501 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1561 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1621 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1681 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1741 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1801 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1861 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1921 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     1981 caatctaaag tatatatgag taaacttggt ctgacagtta gaaaaactca tcgagcatca
     2041 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
     2101 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
     2161 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
     2221 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
     2281 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
     2341 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
     2401 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
     2461 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
     2521 ttttcccagg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
     2581 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
     2641 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     2701 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     2761 catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt
     2821 gaatatggct catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2881 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2941 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     3001 ataaaaatag gcgtatcacg aggccctttc gtc
//
