LOCUS pOTTC1484___pAAV 6069 bp ds-DNA circular 23-MAR-2017 SOURCE ORGANISM COMMENT pOTTC1484 was constructed by Chris Richie at GE&VVC/NIDA (CR1712_4). COMMENT CR1712 - pAAV SYN1 HA-hM4D(Gi) COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin 5344..6026 /label=ColE1 origin /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 686..2155 /label=HA-hM4D(Gi) /ApEinfo_fwdcolor="#0080c0" /ApEinfo_revcolor="#0080c0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 3564..3870 /label=f1 origin /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" protein_bind 689..715 /label=HA epitope [YPYDVPDYA] /ApEinfo_fwdcolor="#ff8080" /ApEinfo_revcolor="#3cbfff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin 3547..4002 /label=M13 origin /ApEinfo_fwdcolor="gray50" /ApEinfo_revcolor="gray50" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 4290..4388 /label=Bla promoter (prokaryotic) /ApEinfo_fwdcolor="#804000" /ApEinfo_revcolor="#808040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" LTR complement(3332..3472) /label=ITR (AAV) /ApEinfo_fwdcolor="#804000" /ApEinfo_revcolor="#ff8000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" LTR 1..141 /label=ITR (AAV)(1) /ApEinfo_label="ITR (AAV)" /ApEinfo_fwdcolor="#804000" /ApEinfo_revcolor="#ff8000" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 156..640 /label=SYN1 promoter /ApEinfo_fwdcolor="#804000" /ApEinfo_revcolor="#808040" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 4389..5246 /label=AmpR /ApEinfo_fwdcolor="#0080c0" /ApEinfo_revcolor="#ff80c0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 2815..3295 /label=hGH polyA /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="#004080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_RNA 2186..2794 /label=WPRE /ApEinfo_fwdcolor="#800080" /ApEinfo_revcolor="#8080c0" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind 597..616 /note=CR1118 /label=hSYN1p F442 /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" primer_bind complement(2307..2326) /note=For OTTC genotyping /label=WPRE R1 /ApEinfo_fwdcolor="steel blue" /ApEinfo_revcolor="orange" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" variation 733..733 /label=Proline silent (T to A) /ApEinfo_fwdcolor="#808080" /ApEinfo_revcolor="#808080" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC 61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA 121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTGTGTC TAGACTGCAG AGGGCCCTGC 181 GTATGAGTGC AAGTGGGTTT TAGGACCAGG ATGAGGCGGG GTGGGGGTGC CTACCTGACG 241 ACCGACCCCG ACCCACTGGA CAAGCACCCA ACCCCCATTC CCCAAATTGC GCATCCCCTA 301 TCAGAGAGGG GGAGGGGAAA CAGGATGCGG CGAGGCGCGT GCGCACTGCC AGCTTCAGCA 361 CCGCGGACAG TGCCTTCGCC CCCGCCTGGC GGCGCGCGCC ACCGCCGCCT CAGCACTGAA 421 GGCGCGCTGA CGTCACTCGC CGGTCCCCCG CAAACTCCCC TTCCCGGCCA CCTTGGTCGC 481 GTCCGCGCCG CCGCCGGCCC AGCCGGACCG CACCACGCGA GGCGCGAGAT AGGGGGGCAC 541 GGGCGCGACC ATCTGCGCTG CGGCGCCGGC GACTCAGCGC TGCCTCAGTC TGCGGTGGGC 601 AGCGGAGGAG TCGTGTCGTG CCTGAGAGCG CAGTCGAGAA GGTACCGGAT CCGAAGTTAT 661 CGCTAGCTCT AGAGTCGACG CCACCATGTA CCCATACGAT GTTCCAGATT ACGCTATGGC 721 CAACTTCACA CCaGTCAATG GCAGCTCGGG CAATCAGTCC GTGCGCCTGG TCACGTCATC 781 ATCCCACAAT CGCTATGAGA CGGTGGAAAT GGTCTTCATT GCCACAGTGA CAGGCTCCCT 841 GAGCCTGGTG ACTGTCGTGG GCAACATCCT GGTGATGCTG TCCATCAAGG TCAACAGGCA 901 GCTGCAGACA GTCAACAACT ACTTCCTCTT CAGCCTGGCG TGTGCTGATC TCATCATAGG 961 CGCCTTCTCC ATGAACCTCT ACACCGTGTA CATCATCAAG GGCTACTGGC CCCTGGGCGC 1021 CGTGGTCTGC GACCTGTGGC TGGCCCTGGA CTGCGTGGTG AGCAACGCCT CCGTCATGAA 1081 CCTTCTCATC ATCAGCTTTG ACCGCTACTT CTGCGTCACC AAGCCTCTCA CCTACCCTGC 1141 CCGGCGCACC ACCAAGATGG CAGGCCTCAT GATTGCTGCT GCCTGGGTAC TGTCCTTCGT 1201 GCTCTGGGCG CCTGCCATCT TGTTCTGGCA GTTTGTGGTG GGTAAGCGGA CGGTGCCCGA 1261 CAACCAGTGC TTCATCCAGT TCCTGTCCAA CCCAGCAGTG ACCTTTGGCA CAGCCATTGC 1321 TGGCTTCTAC CTGCCTGTGG TCATCATGAC GGTGCTGTAC ATCCACATCT CCCTGGCCAG 1381 TCGCAGCCGA GTCCACAAGC ACCGGCCCGA GGGCCCGAAG GAGAAGAAAG CCAAGACGCT 1441 GGCCTTCCTC AAGAGCCCAC TAATGAAGCA GAGCGTCAAG AAGCCCCCGC CCGGGGAGGC 1501 CGCCCGGGAG GAGCTGCGCA ATGGCAAGCT GGAGGAGGCC CCCCCGCCAG CGCTGCCACC 1561 GCCACCGCGC CCCGTGGCTG ATAAGGACAC TTCCAATGAG TCCAGCTCAG GCAGTGCCAC 1621 CCAGAACACC AAGGAACGCC CAGCCACAGA GCTGTCCACC ACAGAGGCCA CCACGCCCGC 1681 CATGCCCGCC CCTCCCCTGC AGCCGCGGGC CCTCAACCCA GCCTCCAGAT GGTCCAAGAT 1741 CCAGATTGTG ACGAAGCAGA CAGGCAATGA GTGTGTGACA GCCATTGAGA TTGTGCCTGC 1801 CACGCCGGCT GGCATGCGCC CTGCGGCCAA CGTGGCCCGC AAGTTCGCCA GCATCGCTCG 1861 CAACCAGGTG CGCAAGAAGC GGCAGATGGC GGCCCGGGAG CGCAAAGTGA CACGAACGAT 1921 CTTTGCCATT CTGCTTGCCT TCATCCTCAC CTGGACGCCC TACAACGTCA TGGTCCTGGT 1981 GAACACCTTC TGCCAGAGCT GCATCCCTGA CACGGTGTGG TCCATTGGCT ACTGGCTCTG 2041 CTACGTCAAC AGCACCATCA ACCCTGCCTG CTATGCTCTG TGCAACGCCA CCTTTAAAAA 2101 GACCTTCCGG CACCTGCTGC TGTGCCAGTA TCGGAACATC GGCACTGCCA GGTAGGGCGC 2161 GCCATAACTT CGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT 2221 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG 2281 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT 2341 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG 2401 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC 2461 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG 2521 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT 2581 TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTA TGTTGCCACC TGGATTCTGC 2641 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG 2701 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA 2761 TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGAGCGCT GCTCGAGAGA TCTACGGGTG 2821 GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC 2881 CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA 2941 TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG 3001 TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC 3061 TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG 3121 ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT 3181 CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC 3241 TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT 3301 GTAGGTAACC ACGTGCGGAC CGAGCGGCCG CAGGAACCCC TAGTGATGGA GTTGGCCACT 3361 CCCTCTCTGC GCGCTCGCTC GCTCACTGAG GCCGGGCGAC CAAAGGTCGC CCGACGCCCG 3421 GGCTTTGCCC GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA GGGGCGCCTG 3481 ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATACG TCAAAGCAAC 3541 CATAGTACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 3601 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 3661 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 3721 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTTGGGTGAT GGTTCACGTA 3781 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 3841 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGGC TATTCTTTTG 3901 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 3961 AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTTATGGTGC ACTCTCAGTA 4021 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG 4081 CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG 4141 GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC 4201 TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG 4261 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT 4321 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA 4381 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT 4441 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT 4501 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT 4561 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG 4621 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA 4681 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA 4741 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA 4801 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA 4861 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA 4921 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA 4981 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC 5041 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC 5101 GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG 5161 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA 5221 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT 5281 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA 5341 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG 5401 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA 5461 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT 5521 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC 5581 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA 5641 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA 5701 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC 5761 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA 5821 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA 5881 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG 5941 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC 6001 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG 6061 CTCACATGT //