LOCUS       TP21_peAAV-CKII(0.4)-Ch 6260 bp ds-DNA     circular     17-DEC-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             2297..2317
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     rep_origin      5611..6199
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    3035..3438
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        4475..4579
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      3738..4193
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             1517..2227
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4580..4648
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      5535..6217
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      3755..4061
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      3738..4193
                     /label="f1 ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        156..518
                     /label="a-CaMKII(0.4)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             2237..2293
                     /label="P2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     repeat_region   3523..3663
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     polyA_signal    3445..3493
                     /label="poly(A) signal"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             2318..3013
                     /label="mScarlet"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             1514..1516
                     /label="EGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4649..5440
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             539..1498
                     /label="Chronos"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2228..2236
                     /label="tinylinker"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          533..550
                     /label="MP_BClI_Chronos_f_(633)"
                     /note="sequence: GACTGATCAgccacaatgacccacgcc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2993..3015)
                     /label="Asc_eYFP_rev_(223)"
                     /note="sequence: GACCAGGGCGCGCCTAATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2204..2227)
                     /label="RFP_o_lox66_eYFP_rev_(238)"
                     /note="sequence: GCCCATGGTGGCGGCGCGCCTGCTGTTACCGTTCGTATAATGTATGCTATACGAAGTTATTCATTACTTGTACAGCTCGTCCATGCCGAG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2318..2336)
                     /label="N_eYFP_rev_(239_3)"
                     /note="sequence: CTCCTCGCCCTTGCTCACCAT"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2989..3009)
                     /label="MP_AscI_mSarlet_for_(659)"
                     /note="sequence: CAGGGCGCGCCTTACTTATACAGCTCGTCCATGCCgccg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(2989..3009)
                     /label="MP_AscI_mSca_for_(654)"
                     /note="sequence: CAGGGCGCGCCctacttatacagctcgtccatgccgccg"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1514..1537
                     /label="eYFP_for_(175)"
                     /note="sequence: GTGAGCAAGGGCGAGGAGCTGTTC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2993..3015)
                     /label="EcoRI_eYFP_rev_(34)"
                     /note="sequence: GACGAGGAATTCTTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          79..94
                     /label="Pri23_olg2_ITR_AAV_titer_r"
                     /note="sequence: CGGCCTCAGTGAGCGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2206..2227)
                     /label="YP29_mScarlet_rev2"
                     /note="sequence: cttgtacagctcgtccatgccg"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2993..3013)
                     /label="AscI_eYFP_rev_(270)"
                     /note="sequence: GGCGCGCCTTATCAGGTCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1972..1992)
                     /label="YP18_seq"
                     /note="sequence: ccgttcttctgcttgtcggcc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1846..1866)
                     /label="YP17_seq"
                     /note="sequence: GTGTCGCCCTCGAACTTCACC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2206..2227)
                     /label="BspEI_eYFP_rev_(487)"
                     /note="sequence: GACTCCGGAttacttgtacagctcgtccatgccg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1755..1777
                     /label="YP14_seq"
                     /note="sequence: ACGACTTCTTcAAGTCCGCCATG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2207..2227)
                     /label="NheI_eYFP_STOP_rev_(145)"
                     /note="sequence: gaccaggctagctaattacttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(115..141)
                     /label="Pri2_D581_peAAV_DIO_WPRE_REV (IA8)"
                     /note="sequence: acttgcactcatacgcagggccctctgcagaggaacccctagtgatggagttggcca"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2972..2993)
                     /label="AscI_mRFP_rev_(251)"
                     /note="sequence: GACCAGGGCGCGCCTCATTATCTGGCGCCGGTGGAGTGGCGGCCCTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1504..1523)
                     /label="EcoR_Arch_rev_(231)"
                     /note="sequence: GCAAGCGAATTCTTATCACGCCTTGCTCACTGCTACTACC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1514..1528
                     /label="MP_P2A_NLS_mSca_f_(635)"
                     /note="sequence: gtggaagaaaaccccggtcctgacccaaaaaagaagcggaaggtcatggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(3467..3493)
                     /label="MP_MluI_polyA_rev_(667)"
                     /note="sequence: CAGACGCGTCACACAAAAAACCAACACACATCCATC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1514..1529
                     /label="YP28_mScarlet_fwd2"
                     /note="sequence: atggtgagcaagggcgagg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2207..2293)
                     /label="MP_AscI_Con_GP2A_r_(634)"
                     /note="sequence: aggaccggggttttcttccacgtctcctgcttgctttaacagagagaagttcgtggctccggatcccttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2204..2225)
                     /label="linker_Arch_rev_(165)"
                     /note="sequence: TGGACCTGGACCAATGTACAGCTCGTCCATGCCGAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2993..3015)
                     /label="MfeI_LOVtoxin_rev_(254)"
                     /note="sequence: GCAGACCAATTGCTATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2195..2229)
                     /label="GPI_A_CNG_eYFP_rev_(97)"
                     /note="sequence: CGGGGTAGCAAGCCAGCACCGTTGCAGAGTGCACCTCCATTACCATCCCCGTTGCCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1502..1523)
                     /label="pL_o_Arch_for_(176)"
                     /note="sequence: CAGAGGTTGATTATCGATAAGCTTTTATCACGCCTTGCTCACTGCTACTACCGG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2228..2245
                     /label="MP_Bsp1407I_GP2A_rev_(653)"
                     /note="sequence: TGTACAGCATTggatccggagccacgaac"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2195..2229)
                     /label="P2A_eCFP_rev_(236)"
                     /note="sequence: GGGACCGGGGTTTTCTTCCACGTCTCCTGCTTGCTTTAACAGAGAGAAGTTCGTGGCTCCGGAGCCCTTGTACAGCTCGTCCATGCCGAGAGTGATCCC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2207..2227)
                     /label="eYFP_AgeI_rev_(1)"
                     /note="sequence: ACCGGTCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          1514..1549
                     /label="BamHI_SecSig_eYFP_for_(17)"
                     /note="sequence: GACTATGGATCCATGTCTGCACTTCTGATCCTAGCTCTTGTTGGAGCTGCAGTTGCTGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1514..1528
                     /label="mGFP_o_ChRI_for_(621)"
                     /note="sequence: gaggaggACgaggacacagtgATGGTGAGCAAGGGCGAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1514..1536
                     /label="eYFP_for_(60)"
                     /note="sequence: gtgagcaagggcgaggagctgtt"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(1584..1603)
                     /label="seq_eYFP_Ct_for_(328)"
                     /note="sequence: GGACACGCTGAACTTGTGGC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(2977..2993)
                     /label="linker_mRFP_rev_(207)"
                     /note="sequence: GGCAGCAGGTGCAGCAGGTGCTGCTCTGGCGCCGGTGGAGTGGCGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2231..2248
                     /label="AscI_hM4D_rev_(420)"
                     /note="sequence: GACACGGGCGCGCCAggctccggagccacgaacttc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1514..1537
                     /label="AscI_eCFP_for_(142)"
                     /note="sequence: gcaacgggcgcgcgccaccatggtgagcaagggcgaggagctgttc"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(2320..2336)
                     /label="D548_pAAV-mDlx-hGtACR2-EYFP [162-1000] REV"
                     /note="sequence: CCCTTGCTCACCATggtggcCCTCCTCGCCCTTGCTCACC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2993..3015)
                     /label="Ecor_LOVtoxin_rev_(234)"
                     /note="sequence: GCAGACGAATTCCTATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1514..1537
                     /label="VVD_o_eYFP_for_(32)"
                     /note="sequence: ATGGGCTTCCAGTGCGAGACAGAGGTGAGCAAGGGCGAGGAGCTGTTC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1514..1537
                     /label="NheI_eYFP_for_(144)"
                     /note="sequence: gacatggctagcgccaccatggtgagcaagggcgaggagctgttc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(3495..3513)
                     /label="MP_aPAAA_rev_(670)"
                     /note="sequence: cggtccgcacgtgggttac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          1514..1537
                     /label="PDFG_eYFP_for_(19)"
                     /note="sequence: ATGCTTTGGCAGAAGAAACCACGTGCTAGCGTGAGCAAGGGCGAGGAGCTGTTC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2335..2362
                     /label="MP_AscI_mSarlet_for_(684)"
                     /note="sequence: TACGGCGCGCCTTGGGCATGGCACCGGCAGCACCGGCAGCGGCAGCTCCGGCACCGCCTCCTCCGAGGACAACAACATGGCAgtgatcaaggagttcatgcggttc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2204..2227)
                     /label="beta_o_eYFP_rev_(171)"
                     /note="sequence: CTGGCTGCATGACTTCTTCTCCTGCTTGTACAGCTCGTCCATGCCGAG"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2318..2336
                     /label="YP28_mScarlet_fwd2"
                     /note="sequence: atggtgagcaagggcgagg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2208..2228)
                     /label="m_turq_o_ChRI_rev_(568)"
                     /note="sequence: gcagggctgccttgtacagctcgtccatgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2277..2293)
                     /label="MP_Gp2a_for_(643)"
                     /note="sequence: gttggccataggaccggggttttctt"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(120..140)
                     /label="Pri22_olg1_ITR_AAV_titer_f"
                     /note="sequence: GGAACCCCTAGTGATGGAGTT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2972..2993)
                     /label="EcoRI_RFP_rev_(198)"
                     /note="sequence: AGCATGGAATTCTCATTATCTGGCGCCGGTGGAGTGGCGGCCCTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1514..1534
                     /label="BamHI_eCFP_for_(237)"
                     /note="sequence: GCAACTGGATCCGCCACCATGGTGAGCAAGGGCGAGGAGCTG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2993..3013)
                     /label="eT2A_mTurq_rev_(555)"
                     /note="sequence: GCCACATGTCAGCAGGCTGCCTCTGCCCTCGCCAGATCCcttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2993..3015)
                     /label="NheI_eYFP_STOP_rev_(145)"
                     /note="sequence: gaccaggctagctaattacttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          2257..2292
                     /label="p2a_GtACR2_for_(614)"
                     /note="sequence: aaagcaagcaggagacgtggaagaaaaccccggtcc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2317..2332
                     /label="eYFP_for_(43)"
                     /note="sequence: GCCACCATGGTGAGCAAGGGC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2207..2229)
                     /label="MP_AscI_Sca_noSTOP_rev_(682)"
                     /note="sequence: TTAGGCGCGCCcttgtacagctcgtccatGCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1514..1534
                     /label="RFC_TRPV_eYFP_(307)"
                     /note="sequence: GAGAAGATGGGCTTCCAGTGCGAGATGGTGAGCAAGGGCGAGGAGCTG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(2207..2227)
                     /label="EcoRI_eYFP_rev_(34)"
                     /note="sequence: GACGAGGAATTCTTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1514..1529
                     /label="hChR2_o_TaqRFP_for_(601)"
                     /note="sequence: ccgaggccggagccgtgccaATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2993..3013)
                     /label="MP_AscI_Con_GP2A_r_(634)"
                     /note="sequence: aggaccggggttttcttccacgtctcctgcttgctttaacagagagaagttcgtggctccggatcccttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(24..39)
                     /label="Pri23_olg2_ITR_AAV_titer_r"
                     /note="sequence: CGGCCTCAGTGAGCGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          3625..3640
                     /label="Pri23_olg2_ITR_AAV_titer_r"
                     /note="sequence: CGGCCTCAGTGAGCGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2993..3013)
                     /label="MP_AscI_Sca_noSTOP_rev_(682)"
                     /note="sequence: TTAGGCGCGCCcttgtacagctcgtccatGCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2207..2227)
                     /label="Asc_eYFP_rev_(223)"
                     /note="sequence: GACCAGGGCGCGCCTAATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2993..3013)
                     /label="MP_eGFP_o_beta_rev_(646)"
                     /note="sequence: cttctcctgcagcttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2273..2335
                     /label="MP_P2A_NLS_mSca_f_(635)"
                     /note="sequence: gtggaagaaaaccccggtcctgacccaaaaaagaagcggaaggtcatggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(2207..2227)
                     /label="Ecor_LOVtoxin_rev_(234)"
                     /note="sequence: GCAGACGAATTCCTATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2992..3015)
                     /label="BspEI_eYFP_rev_(487)"
                     /note="sequence: GACTCCGGAttacttgtacagctcgtccatgccg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          533..550
                     /label="MP_NcoI_Chronos_for_(671)"
                     /note="sequence: CAGCCATGGgccacaatgacccacgcc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3570..3585)
                     /label="Pri23_olg2_ITR_AAV_titer_r"
                     /note="sequence: CGGCCTCAGTGAGCGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(979..994)
                     /label="a554c_antisense_(332)"
                     /note="sequence: tcttgttgtagtcgtttgccagtccggtcaggttg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(2207..2227)
                     /label="AscI_eYFP_rev_(270)"
                     /note="sequence: GGCGCGCCTTATCAGGTCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3524..3544
                     /label="Pri22_olg1_ITR_AAV_titer_f"
                     /note="sequence: GGAACCCCTAGTGATGGAGTT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2207..2229)
                     /label="p2a_o_TagRFP_rev_(602)"
                     /note="sequence: aagttcgtggctccggagccCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2993..3013)
                     /label="p2a_o_TagRFP_rev_(602)"
                     /note="sequence: aagttcgtggctccggagccCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2207..2227)
                     /label="MP_eGFP_o_beta_rev_(646)"
                     /note="sequence: cttctcctgcagcttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          3523..3549
                     /label="Pri2_D581_peAAV_DIO_WPRE_REV (IA8)"
                     /note="sequence: acttgcactcatacgcagggccctctgcagaggaacccctagtgatggagttggcca"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2993..3015)
                     /label="NheI_mCherry_for_(377)"
                     /note="sequence: ACGAATGCTAGCttacttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          2339..2362
                     /label="MP_dt_mScarlet_for_(658)"
                     /note="sequence: CAGTGTACATTGGGCATGGCACCGGCAGCACCGGCAGCGGCAGCTCCGGCACCGCCTCCTCCGAGGACAACAACATGGCCgtgatcaaggagttcatgcggttc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2318..2338
                     /label="MP_rev_(714)"
                     /note="sequence: atggtgagcaagggcgaggca"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(3474..3493)
                     /label="seq_NaN_(632)"
                     /note="sequence: tcaaaTGACACACAAAAAACCAACACAC"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2318..2336
                     /label="hChR2_o_TaqRFP_for_(601)"
                     /note="sequence: ccgaggccggagccgtgccaATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2318..2335
                     /label="mGFP_o_ChRI_for_(621)"
                     /note="sequence: gaggaggACgaggacacagtgATGGTGAGCAAGGGCGAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2217..2245
                     /label="MP_Gp2a_rev_(642)"
                     /note="sequence: agctgtacaagggatccggagccacgaac"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3472..3493)
                     /label="707_smallpA_for_MP"
                     /note="sequence: TCACGGACCGCAATTGCACACAAAAAACCAACACACAT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2941..2962
                     /label="MP_mScarlet_seq_rev_(665)"
                     /note="sequence: ctacaccgtggtggaacagtac"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          526..550
                     /label="MP_KpnI_Chronos_f_(651)"
                     /note="sequence: GACGGTACCgccacaatgacccacgcc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(2207..2227)
                     /label="MfeI_LOVtoxin_rev_(254)"
                     /note="sequence: GCAGACCAATTGCTATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2231..2250
                     /label="p2a_for_(371)"
                     /note="sequence: tccggagccacgaacttctc"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2992..3013)
                     /label="YP29_mScarlet_rev2"
                     /note="sequence: cttgtacagctcgtccatgccg"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(3472..3493)
                     /label="704_Mfe_smallpA_rev_MP"
                     /note="sequence: GAACAATTGCACACAAAAAACCAACACACAT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(2207..2232)
                     /label="eT2A_mTurq_rev_(555)"
                     /note="sequence: GCCACATGTCAGCAGGCTGCCTCTGCCCTCGCCAGATCCcttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(3472..3493)
                     /label="698_smallpA_for_MP"
                     /note="sequence: tgcaattgttgttgttCACACAAAAAACCAACACACAT"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(3467..3493)
                     /label="695_minPolyA_Mlu_MP"
                     /note="sequence: GACACGCGTCACACAAAAAACCAACACACATCCATC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(1505..1523)
                     /label="RFC_Arch_pLenti_(308)"
                     /note="sequence: AGGTTGATTATCGATAAGCTTGATATCGAATTCTTATCACGCCTTGCTCACTGCTACTAC"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2228..2245
                     /label="MP_AscI_GP2A_rev_(683)"
                     /note="sequence: TACGGCGCGCCAggatccggagccacgaac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2210..2227)
                     /label="BamHI_eyFP_rev_(51)"
                     /note="sequence: gcatagggatccttacttgtacagctcgtccat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3418..3436)
                     /label="seq_WPRE_upstream_rev_(347)"
                     /note="sequence: CGTCAGCTTCCCCGACAAC"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          353..372
                     /label="Pri32_S4_seq (MP13)"
                     /note="sequence: cgtttcggaggtggttgcca"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2207..2227)
                     /label="NheI_mCherry_for_(377)"
                     /note="sequence: ACGAATGCTAGCttacttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          3035..3057
                     /label="703_smallWPRE_for_MP"
                     /note="sequence: gagctgtacaagtagAATCAACCTCTGGATTACAAAAT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2941..2962)
                     /label="MP_mScarlet_seq_for_(664)"
                     /note="sequence: gtactgttccaccacggtgtag"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2256..2278
                     /label="p2a_seq_rev_(296)"
                     /note="sequence: taaagcaagcaggagacgtggaa"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2297..2317
                     /label="Pri25_OL555_f (MP7)"
                     /note="sequence: agtgccatgggccacaatgaCCAAAAAAGAAGCGGAAGGTC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2991..3015)
                     /label="pL_o_mCherry_rev_(256)"
                     /note="sequence: GTAATCCAGAGGTTGATTATCGATAAGCTTGATATCGAATTCTTACTTGTACAGCTCGTCCATGCCGC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3027..3047
                     /label="697_smallWPRE_for_MP"
                     /note="sequence: tcaagcttAATCAACCTCTGG"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          2256..2278
                     /label="p2a_seq_(295)"
                     /note="sequence: taaagcaagcaggagacgtggaa"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2993..3013)
                     /label="eYFP_AgeI_rev_(1)"
                     /note="sequence: ACCGGTCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(2996..3015)
                     /label="BamHI_eyFP_rev_(51)"
                     /note="sequence: gcatagggatccttacttgtacagctcgtccat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(1495..1513)
                     /label="CoChR2_rev_(553)"
                     /note="sequence: cttggacaccattgctactaccggtgccgcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2994..3013)
                     /label="m_turq_o_ChRI_rev_(568)"
                     /note="sequence: gcagggctgccttgtacagctcgtccatgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtcttgt ggactaagtt tgttcgcatc
      181 cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt gctcctgggc
      241 ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca ggttttaaag
      301 tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag ggcgtttcgg
      361 aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg agcagccaca
      421 gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt gcactcagga
      481 gcacgggcag gcgagtggcc cctagttctg ggggcagctc tagagcggta ccgccacaat
      541 gacccacgcc tttatctcag ccgtgcctag cgccgaagcc acaattagag gcctgctgag
      601 cgccgcagca gtggtgacac cagcagcaga cgctcacgga gaaacctcta acgccacaac
      661 agccggagcc gatcacggtt gcttccccca catcaaccac ggaaccgagc tgcagcacaa
      721 gatcgcagtg ggactccagt ggttcaccgt gatcgtggct atcgtgcagc tcatcttcta
      781 cggttggcac agcttcaagg ccacaaccgg ctgggaggag gtctacgtct gcgtgatcga
      841 gctcgtcaag tgcttcatcg agctgttcca cgaggtcgac agcccagcca cagtgtacca
      901 gaccaacgga ggagccgtga tttggctgcg gtacagcatg tggctcctga cttgccccgt
      961 gatcctgatc cacctgagca acctgaccgg actgcacgaa gagtacagca agcggaccat
     1021 gaccatcctg gtgaccgaca tcggcaacat cgtgtggggg atcacagccg cctttacaaa
     1081 gggccccctg aagatcctgt tcttcatgat cggcctgttc tacggcgtga cttgcttctt
     1141 ccagatcgcc aaggtgtata tcgagagcta ccacaccctg cccaaaggcg tctgccggaa
     1201 gatttgcaag atcatggcct acgtcttctt ctgctcttgg ctgatgttcc ccgtgatgtt
     1261 catcgccgga cacgagggac tgggcctgat cacaccttac accagcggaa tcggccacct
     1321 gatcctggat ctgatcagca agaacacttg gggcttcctg ggccaccacc tgagagtgaa
     1381 gatccacgag cacatcctga tccacggcga catccggaag acaaccacca tcaacgtggc
     1441 cggcgagaac atggagatcg agaccttcgt cgacgaggag gaggagggag gagtggcggc
     1501 accggtagta gcagtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1561 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1621 tgccacctac ggcaagctga ccctgaagtt catttgcacc accggcaagc tgcccgtgcc
     1681 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     1741 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     1801 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1861 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1921 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     1981 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2041 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2101 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2161 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2221 gtacaaggga tccggagcca cgaacttctc tctgttaaag caagcaggag acgtggaaga
     2281 aaaccccggt cctgacccaa aaaagaagcg gaaggtcatg gtgagcaagg gcgaggcagt
     2341 gatcaaggag ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt
     2401 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     2461 ggtgaccaag ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta
     2521 cggctccagg gccttcacca agcaccccgc cgacatcccc gactactata agcagtcctt
     2581 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt
     2641 gacccaggac acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac
     2701 caacttccct cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcgtccac
     2761 cgagcggttg taccccgagg acggcgtgct gaagggcgac attaagatgg ccctgcgcct
     2821 gaaggacggc ggacgctacc tggcggactt caagaccacc tacaaggcca agaagcccgt
     2881 gcagatgccc ggcgcctaca acgtcgaccg caagttggac atcacctccc acaacgagga
     2941 ctacaccgtg gtggaacagt acgaacgctc cgagggccgc cactccaccg gcggcatgga
     3001 cgagctgtac aagtagGAAT Tcgatatcaa gcttAATCAA CCTCTGGATT ACAAAATTTG
     3061 TGAAAGATTG ACTGATATTC TTAACTATGT TGCTCCTTTT ACGCTGTGTG GATATGCTGC
     3121 TTTAATGCCT CTGTATCATG CTATTGCTTC CCGTATGGCT TTCGTTTTCT CCTCCTTGTA
     3181 TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCCGTC AACGTGGCGT
     3241 GGTGTGCTCT GTGTTTGCTG ACGCAACCCC CACTGGCTGG GGCATTGCCA CCACCTGTCA
     3301 ACTCCTTTCT GGGACTTTCG CTTTCCCCCT CCCGATCGCC ACGGCAGAAC TCATCGCCGC
     3361 CTGCCTTGCC CGCTGCTGGA CAGGGGCTAG GTTGCTGGGC ACTGATAATT CCGTGGTGTT
     3421 GTCGGGGAAG CTGACGTCag cgctAATAAA ATCGCTATCC ATCGAAGATG GATGTGTGTT
     3481 GGTTTTTTGT GTGggtaacc cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     3541 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     3601 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     3661 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     3721 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     3961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     4081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4141 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4201 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4261 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4321 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4381 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4441 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4501 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4561 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4621 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4681 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4741 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4801 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4861 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4921 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4981 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5041 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5101 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5161 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5221 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5281 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5341 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5401 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5461 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5521 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     5761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6001 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6241 ctggcctttt gctcacatgt
//