LOCUS       TP22_pAAV-EF1-fDIO-mNeo 5584 bp ds-DNA     circular     02-JUN-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    726..759
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    1..141
                     /label="L-ITR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        156..701
                     /label="EF1 promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2847..2987
                     /label="R-ITR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    810..843
                     /label="F5"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      4912..5579
                     /label="pUC ori"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    complement(1653..1686)
                     /label="F5"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      3079..3385
                     /label="f1 Origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    complement(1569..1602)
                     /label="FRT (minimal)"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     misc_feature    1701..2309
                     /label="WPRE"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     polyA_signal    2329..2807
                     /label="hGH polyA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(855..1562)
                     /label="mNeonGreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3904..4761
                     /label="AmpR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(1223..1249)
                     /label="694_mNeon_seq_for_MP"
                     /note="sequence: CCTCCCTTACTGTTAACTACCGCTACA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          858..878
                     /label="MP_eGFP_o_beta_rev_(646)"
                     /note="sequence: cttctcctgcagcttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          858..878
                     /label="eT2A_mTurq_rev_(555)"
                     /note="sequence: GCCACATGTCAGCAGGCTGCCTCTGCCCTCGCCAGATCCcttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          858..878
                     /label="MP_AscI_Sca_noSTOP_rev_(682)"
                     /note="sequence: TTAGGCGCGCCcttgtacagctcgtccatGCC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2845..2865
                     /label="Project4_MP_pAAV_DLXbeta_nls-mScarlet-WPRE FWD"
                     /note="sequence: GTGTGTTGGTTTTTTGTGTGcaggaacccctagtgatgga"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          join(5566..5584,1..1)
                     /label="Pri36_EF1_for"
                     /note="sequence: GTTCTGCGCCGTTACAGATC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2530..2554
                     /label="Pri34_seq_PolyA_f"
                     /note="sequence: tctattgggaaccaagctggagtgc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(120..140)
                     /label="Pri22_olg1_ITR_AAV_titer_f"
                     /note="sequence: GGAACCCCTAGTGATGGAGTT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          858..877
                     /label="m_turq_o_ChRI_rev_(568)"
                     /note="sequence: gcagggctgccttgtacagctcgtccatgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          854..877
                     /label="D265_AAV-CKIIa(0.4)-mNeonGreen-minBack REV"
                     /note="sequence: TAATCCAGAGGTTGATTctaTTACTTGTACAGCTCGTCCATGC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2847..2868
                     /label="Pri21_2_D581_peAAV_DIO_WPRE_REV(IA8)"
                     /note="sequence: acttgcactcatacgcagggccctctgcagaggaacccctagtgatggagtt"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          661..680
                     /label="Pri36_EF1_for"
                     /note="sequence: GTTCTGCGCCGTTACAGATC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          858..878
                     /label="AscI_eYFP_rev_(270)"
                     /note="sequence: GGCGCGCCTTATCAGGTCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          844..878
                     /label="Asc_eYFP_rev_(223)"
                     /note="sequence: GACCAGGGCGCGCCTAATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1547..1562)
                     /label="Pri8_D555_mScarlet_REV(IA14)"
                     /note="sequence: gtcgacgaggaggaggagggaggagtggcgatggtgagcaagggcgagg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(120..141)
                     /label="Pri21_2_D581_peAAV_DIO_WPRE_REV(IA8)"
                     /note="sequence: acttgcactcatacgcagggccctctgcagaggaacccctagtgatggagtt"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2949..2964
                     /label="Pri23_olg2_ITR_AAV_titer_r"
                     /note="sequence: CGGCCTCAGTGAGCGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2835..2858
                     /label="Pri38_Project4_MP_pAAV_DLXbeta_nls-mScarlet-WPRE REV"
                     /note="sequence: GCCCTCTGCAGTCTAGACACgcagtgaaaaaaatgctttatttg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1223..1249
                     /label="693_mNeon_seq_rev_MP"
                     /note="sequence: TGTAGCGGTAGTTAACAGTAAGGGAGG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          1711..1733
                     /label="703_smallWPRE_for_MP"
                     /note="sequence: gagctgtacaagtagAATCAACCTCTGGATTACAAAAT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          858..878
                     /label="p2a_o_TagRFP_rev_(602)"
                     /note="sequence: aagttcgtggctccggagccCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1546..1562)
                     /label="D265_AAV-CKIIa(0.4)-mNeonGreen-minBack FWD"
                     /note="sequence: caaaaaagaagcggaaggtcATGGTGAGCAAGGGCGAGGA"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1548..1562)
                     /label="MP_P2A_NLS_mSca_f_(635)"
                     /note="sequence: gtggaagaaaaccccggtcctgacccaaaaaagaagcggaaggtcatggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(141..155)
                     /label="D581_peAAV_CAG_DIO_dtScarlet REV"
                     /note="sequence: cctctgcagtctagacacacatcacgcgtgcggccgca"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(122..141)
                     /label="D581_peAAV_CAG_DIO_dtScarlet REV"
                     /note="sequence: atacgcagggccctctgcagaggaacccctagtgatggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1693..1716
                     /label="QC_desEcoRI_rev_(471)"
                     /note="sequence: gtatgctatacgaagttatgaattggatatcaagcttatcgataatcaa"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          855..878
                     /label="NheI_mCherry_for_(377)"
                     /note="sequence: ACGAATGCTAGCttacttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          851..878
                     /label="NheI_eYFP_STOP_rev_(145)"
                     /note="sequence: gaccaggctagctaattacttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(1547..1562)
                     /label="YP28_mScarlet_fwd2"
                     /note="sequence: atggtgagcaagggcgagg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          1711..1733
                     /label="D388_peAAV_CKII(1.3)_NES_tag_jRGECO1a FWD"
                     /note="sequence: aaatgatgacagcgaagtgaAATCAACCTCTGGATTACAAAAT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          858..878
                     /label="eYFP_AgeI_rev_(1)"
                     /note="sequence: ACCGGTCTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(115..141)
                     /label="Pri2_D581_peAAV_DIO_WPRE_REV (IA8)"
                     /note="sequence: acttgcactcatacgcagggccctctgcagaggaacccctagtgatggagttggcca"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1548..1562)
                     /label="mGFP_o_ChRI_for_(621)"
                     /note="sequence: gaggaggACgaggacacagtgATGGTGAGCAAGGGCGAG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1547..1562
                     /label="D581_peAAV_CAG_DIO_dtScarlet FWD"
                     /note="sequence: gagataaaggcgtgggtcatCtgcctcgcccttgcTCac"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1544..1562)
                     /label="Pri16_kozak_mNeon_for (DH5)"
                     /note="sequence: gccaccATGGTGAGCAAGGGCGAGGAGG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(130..153)
                     /label="Pri38_Project4_MP_pAAV_DLXbeta_nls-mScarlet-WPRE REV"
                     /note="sequence: GCCCTCTGCAGTCTAGACACgcagtgaaaaaaatgctttatttg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          854..878
                     /label="MfeI_LOVtoxin_rev_(254)"
                     /note="sequence: GCAGACCAATTGCTATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          79..94
                     /label="Pri23_olg2_ITR_AAV_titer_r"
                     /note="sequence: CGGCCTCAGTGAGCGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2847..2866
                     /label="D581_peAAV_CAG_DIO_dtScarlet REV"
                     /note="sequence: atacgcagggccctctgcagaggaacccctagtgatggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(24..39)
                     /label="Pri23_olg2_ITR_AAV_titer_r"
                     /note="sequence: CGGCCTCAGTGAGCGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1107..1127)
                     /label="MP_mNeon_seq_rev_(663)"
                     /note="sequence: GACTGGTGCAGGTCGAAGAAG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(2894..2909)
                     /label="Pri23_olg2_ITR_AAV_titer_r"
                     /note="sequence: CGGCCTCAGTGAGCGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1687..1715
                     /label="Pri28_P5_Chronos_f (MP9)"
                     /note="sequence: ctacgagaacgaggtgtaatgaattcgatatcaagcttatcgataatca"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(122..141)
                     /label="D581_peAAV_CAG_DIO_dtScarlet REV"
                     /note="sequence: atacgcagggccctctgcagaggaacccctagtgatggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(128..147)
                     /label="Pri37_Project4_MP_pAAV_DLXbeta_nls-mScarlet-WPRE FWD"
                     /note="sequence: GTGTGTTGGTTTTTTGTGTGcaggaacccctagtgatgga"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          706..723
                     /label="QC_NheI_rev_(469)"
                     /note="sequence: tatacgaagttatggcgctagcctctagagtcgactccg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          858..878
                     /label="MP_AscI_Con_GP2A_r_(634)"
                     /note="sequence: aggaccggggttttcttccacgtctcctgcttgctttaacagagagaagttcgtggctccggatcccttgtacagctcgtccatgcc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          1711..1733
                     /label="Pri43_D388_peAAV_CKII(1.3)_NES_tag_jRGECO1a FWD"
                     /note="sequence: aatgatgacagcgaagtgaaAATCAACCTCTGGATTACAAAAT"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2847..2873
                     /label="Pri2_D581_peAAV_DIO_WPRE_REV (IA8)"
                     /note="sequence: acttgcactcatacgcagggccctctgcagaggaacccctagtgatggagttggcca"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1699..1722
                     /label="pL_hSyn_for_(159)"
                     /note="sequence: AAGCTTATCGATAATCAACCTCTG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          855..878
                     /label="EcoRI_eYFP_rev_(34)"
                     /note="sequence: GACGAGGAATTCTTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(123..143)
                     /label="Project4_MP_pAAV_DLXbeta_nls-mScarlet-WPRE FWD"
                     /note="sequence: GTGTGTTGGTTTTTTGTGTGcaggaacccctagtgatgga"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          855..875
                     /label="BamHI_eyFP_rev_(51)"
                     /note="sequence: gcatagggatccttacttgtacagctcgtccat"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2841..2860
                     /label="Pri37_Project4_MP_pAAV_DLXbeta_nls-mScarlet-WPRE FWD"
                     /note="sequence: GTGTGTTGGTTTTTTGTGTGcaggaacccctagtgatgga"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1107..1127
                     /label="MP_mNeon_seq_for_(662)"
                     /note="sequence: CTTCTTCGACCTGCACCAGTC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1547..1562)
                     /label="hChR2_o_TaqRFP_for_(601)"
                     /note="sequence: ccgaggccggagccgtgccaATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          792..816
                     /label="MP_F5_seq_for_(669)"
                     /note="sequence: GCAATATGAAACCTCTTAGAAGTTC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          854..878
                     /label="Ecor_LOVtoxin_rev_(234)"
                     /note="sequence: GCAGACGAATTCCTATTACTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          2848..2868
                     /label="Pri22_olg1_ITR_AAV_titer_f"
                     /note="sequence: GGAACCCCTAGTGATGGAGTT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(1765..1786)
                     /label="OS7"
                     /note="sequence: CACATAGCGTAAAAGGAGCAAC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2847..2866
                     /label="D581_peAAV_CAG_DIO_dtScarlet REV"
                     /note="sequence: atacgcagggccctctgcagaggaacccctagtgatggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA CGCGTAAGGA TCTGCGATCG CTCCGGTGCC
      181 CGTCAGTGGG CAGAGCGCAC ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC
      241 AATTGAACGG GTGCCTAGAG AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC
      301 TGGCTCCGCC TTTTTCCCGA GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG
      361 AACGTTCTTT TTCGCAACGG GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC
      421 ATCTCTCCTT CACGCGCCCG CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC
      481 GTTCTGCCGC CTCCCGCCTG TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA
      541 AAGCTCAGGT CGAGACCGGG CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC
      601 CGGCTCTCCA CGCTTTGCCT GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT
      661 GTTCTGCGCC GTTACAGATC CAAGCTGTGA CCGGCGCCTA CGGATCCTCT AGAGTCGACT
      721 CCGGAGAAGT TCCTATTCTC TAGAAAGTAT AGGAACTTCG CAGAATGGTA GCTGGATTGT
      781 AGCTGCTATT AGCAATATGA AACCTCTTAG AAGTTCCTAT TCTTCAAAAG GTATAGGAAC
      841 TTCGGCGCGC CTAATTACTT GTACAGCTCG TCCATGCCCA TCACATCGGT AAAGGCCTTT
      901 TGCCACTCCT TGAAGTTGAG CTCGGTCTTG GAGTGCTTGA GCTCCGTCTT ACGGAACACG
      961 TACATCGGCT GGTTCTTCAG ATAGTTAGCC GCCATTGGCT TGGCAAAGGT GTAGGTGGTC
     1021 CGCGCAGTGC TCCGGTAGCG CTTGCCATTT CCAGTGGTGT AACTCCACTT AAAGGTACTG
     1081 ATGATGGTTT TGTCGTTGGG GTAAGTCTTC TTCGACCTGC ACCAGTCCGC AGCGGTCAGC
     1141 GAGTTGGTCA TCACAGGACC GTCAGCAGGG AAACCAGTCC CCTTCACCTG GGCCTCTCCT
     1201 TTGATGTGGC TTCCCTCGTA GGTGTAGCGG TAGTTAACAG TAAGGGAGGC ACCATCTTCA
     1261 AACTGCATTG TGCGATGGAC TTGGTATCCA GAGCCATCTA CCATAGCGGC CTGGAAAGGC
     1321 GACATCCCGT CAGGGTAGGG CAGGTACTGA TGGAAGCCAT ACCCGATATG AGGGACCAGA
     1381 ATCCAGGGGG AGAACTGGAG GTCgCCCTTG GTGGACTTCA GGTTTAACTC CTCATAACCA
     1441 TCATTTGGAT TGCCGGTGCC CTGACCCACC ATGTCAAAGT CCACACCGTT GATGGAGCCA
     1501 AAGATGTGTA ACTCATGTGT CGCTGGGAGA GAGGCCATGT TATCCTCCTC GCCCTTGCTC
     1561 ACGCTAGCGA AGTTCCTATA CTTTCTAGAG AATAGGAACT TCTTGCCTTA ACCCAGAAAT
     1621 TATCACTGTT ATTCTTTAGA ATGGTGCAAA GAGAAGTTCC TATACCTTTT GAAGAATAGG
     1681 AACTTCGAAT TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA
     1741 AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA
     1801 ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA
     1861 TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG
     1921 TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC
     1981 CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC
     2041 CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG
     2101 GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTATGTTG CCACCTGGAT TCTGCGCGGG
     2161 ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG
     2221 CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC
     2281 CTTTGGGCCG CCTCCCCGCA TCGATACCGA GCGCTGCTCG AGAGATCTAC GGGTGGCATC
     2341 CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA
     2401 GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA
     2461 TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG
     2521 CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA
     2581 TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC
     2641 AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA
     2701 TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA
     2761 AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTGTAGG
     2821 TAACCACGTG CGGACCGAGC GGCCGCAGGA ACCCCTAGTG ATGGAGTTGG CCACTCCCTC
     2881 TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC GCCCGGGCTT
     2941 TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC CTGCAGGGGC GCCTGATGCG
     3001 GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATACGTCAAA GCAACCATAG
     3061 TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC
     3121 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC
     3181 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
     3241 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG
     3301 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT
     3361 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA
     3421 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
     3481 AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT GGTGCACTCT CAGTACAATC
     3541 TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC
     3601 TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC
     3661 TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG
     3721 ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC
     3781 ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT
     3841 ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG
     3901 AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT
     3961 CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT
     4021 GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC
     4081 CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA
     4141 TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC
     4201 TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA
     4261 TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG
     4321 ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC
     4381 CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG
     4441 ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA
     4501 GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG
     4561 CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG
     4621 TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC
     4681 TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT
     4741 GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT
     4801 GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC
     4861 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     4921 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     4981 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     5041 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
     5101 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     5161 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     5221 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     5281 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
     5341 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     5401 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     5461 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     5521 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     5581 ATGT
//